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Aes Antibody (PA5-114846) in ICC/IF
Immunocytochemistry analysis of Aes in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114846) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-114846
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human AES(Accession Q08117), corresponding to amino acid residues M1-L43.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2899482
产品详细信息
Antibody detects endogenous levels of total Amino-terminal enhancer of split.
靶标信息
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Amino enhancer of split; Amino-terminal enhancer of split; Gp130-associated protein GAM; Grg-5; Groucho-related protein 5; Protein ESP1; Protein GRG; R-esp1; related to Drosophila groucho; TLE family member 5; TLE family member 5, transcriptional modulator
基因别名: AES; AES-1; AES-2; AL024115; ESP1; GRG; Grg-5; GRG5; TLE5
UniProt ID: (Human) Q08117, (Mouse) P63002, (Rat) P63003
Entrez Gene ID: (Human) 166, (Mouse) 14797, (Rat) 29466
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
multicellular organism development
organ morphogenesis
negative regulation of gene expression
Wnt signaling pathway
negative regulation of protein binding
negative regulation of transcription, DNA-templated
negative regulation of response to cytokine stimulus
response to interleukin-1
negative regulation of canonical Wnt signaling pathway
positive regulation of anoikis
skeletal system development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular response to extracellular stimulus
regulation of growth
