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产品信息
14-5789-82
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
8B2
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10734227
产品详细信息
Description: This 8B2 monoclonal antibody reacts with mouse Aiolos, a 58-kDa member of the Ikaros family of zinc finger transcription factors. Expression of Aiolos is restricted to the lymphoid lineage, with B cells exhibiting the highest levels of expression, followed by NK and T cells. Studies demonstrate that Aiolos forms homodimers or heterodimerizes with Ikaros proteins. Moreover, Aiolos exhibits tyrosine phosphorylation and nuclear localization in response to IL-2 stimulation in T cells. Aiolos plays a role in IL-2-mediated anti-apoptosis by controlling Bcl-2 expression. Finally, Aiolos is involved in B cell development, with upregulation of this transcription factor observed at the pre-BI to pre-BII transition in response to pre-B cell receptor signaling.
Applications Reported: This 8B2 antibody has been reported for use in immunoblotting/western blotting.
Applications Tested: This 8B2 antibody has been tested by immunoblotting of reduced cell lysate prepared from mouse splenocytes. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Ikaros family zinc finger protein 3; Zinc finger protein Aiolos; zinc finger protein, subfamily 1A, 3 (Aiolos)
基因别名: 5830411O07Rik; Aiolos; Ikzf3; Zfpn1a3; Znfn1a3
UniProt ID: (Mouse) O08900
Entrez Gene ID: (Mouse) 22780
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
DNA binding
protein binding
protein homodimerization activity
sequence-specific DNA binding
metal ion binding
protein heterodimerization activity
C2H2 zinc finger transcription factor
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
regulation of B cell proliferation
B cell activation
regulation of apoptotic process
regulation of B cell differentiation
regulation of lymphocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
