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Invitrogen
Alexa Fluor 488 Recombinant Rabbit Monoclonal Antibody (15H9L93)
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Alexa Fluor 488 Antibody (704060) in ICC/IF
Immunofluorescent analysis of Alexa Fluor™ 488 was performed using 70% confluent HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with H3 Recombinant Rabbit Monoclonal Antibody (Product # 711055, 1:500) at 4 degree celsius overnight and then labeled with... View More
产品信息
704060
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
宿主/亚型
Rabbit
/ IgG
Expression System
ExpiCHO
分类
Recombinant Monoclonal
类型
Antibody
克隆号
15H9L93
抗原
Alexa Fluor® 488 dye conjugated to KLH
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3090464
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes. The Alexa Fluor® 488 dye possesses near identical spectral properties and quantum yield to fluorescein isothiocyanate (FITC), but produces brighter, more photostable conjugates. These conjugates are used for imaging and other applications requiring increased sensitivity without being overly impacted by environmental factors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AF488; Alexa 489; AlexaFluor488
