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产品信息
PA1-009
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Rat
已发表种属
Chicken,
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified alpha-A Crystallin from bovine lens.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 30mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2084139
产品详细信息
PA1-009 detects alpha-A crystallin from bovine and rat samples. This antibody does not detect the unphosphorylated form of the protein.
PA1-009 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~20 kDa protein representing alpha-A crystallin from bovine lens samples. Immunohistochemical staining of alpha-A crystallin in rat thymus samples results in staining of epithelial reticular cells.
The PA1-009 immunogen is purified alpha-A Crystallin from bovine lens.
Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.
靶标信息
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Acry 1; Alpha-crystallin A chain; Alpha-crystallin A(1-151); Alpha-crystallin A(1-156); Alpha-crystallin A(1-157); Alpha-crystallin A(1-162); Alpha-crystallin A(1-163); Alpha-crystallin A(1-165); Alpha-crystallin A(1-168); Alpha-crystallin A(1-172); CRYA 1; Crystallin Alpha 1; Crystallin, alpha polypeptide A; HSPB 4
基因别名: Acry-1; BOS_1386; CRYA1; CRYAA
UniProt ID: (Bovine) P02470, (Rat) P24623
Entrez Gene ID: (Bovine) 281718, (Rat) 24273
obsolete M phase specific microtubule process
response to hypoxia
positive regulation of protein phosphorylation
mitochondrion organization
actin filament organization
tubulin complex assembly
visual perception
negative regulation of gene expression
positive regulation of cell growth
negative regulation of intracellular transport
protein refolding
response to hydrogen peroxide
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
embryonic camera-type eye morphogenesis
protein homooligomerization
apoptotic process involved in morphogenesis
response to UV-A
lens fiber cell morphogenesis
lens development in camera-type eye
lens morphogenesis in camera-type eye
protein folding
apoptotic process
microtubule-based process
response to lead ion
response to drug
camera-type eye development
response to glucocorticoid
