Invitrogen

Alpha A Crystallin Monoclonal Antibody (1H3.B8)

Alpha A Crystallin Antibody in Immunocytochemistry (ICC/IF)

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Alpha A Crystallin Antibody (MA5-27582) in ICC/IF

Immunofluorescent analysis of Alpha A Crystallin in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Alpha A Crystallin monoclonal antibody (Product # MA5-27582) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5... View More

Alpha A Crystallin Antibody in Western Blot (WB)

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产品信息

MA5-27582

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫细胞化学 (ICC/IF)

Assay-Dependent

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Bovine, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1H3.B8

抗原

Native Human Alpha Crystallin

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2735107

产品详细信息

0.5 µg/mL was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects approximately 20kDa. Does not cross-react with alpha B-crystallin, beta L-crystallin, beta H- crystallin, gamma -crystallin, HSP25, HSP27 or HSP47 proteins.

靶标信息

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Acry 1; alpha crystallin A chain; alpha-A-crystallin; Alpha-crystallin A chain; CRYA 1; Crystallin Alpha 1; crystallin, alpha 1; crystallin, alpha A; Crystallin, alpha polypeptide A; crystallin, alpha-1; Heat shock protein beta-4; Heat shock protein family B member 4; HSPB 4; HspB4; human alphaA-crystallin (CRYA1); lens opacity 18

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基因别名: Acry-1; Crya-1; CRYA1; CRYAA; CTRCT9; DAcry-1; HSPB4; lop18