Invitrogen

Alpha A Crystallin Recombinant Rabbit Monoclonal Antibody (ARC1245)

Alpha A Crystallin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Alpha A Crystallin Antibody (MA5-35646) in IHC (P)

Immunohistochemistry (Immunofluorescence) analysis of Alpha A Crystallin in mouse eye. Samples were incubated with Alpha A Crystallin Monoclonal antibody (Product # MA5-35646) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Alpha A Crystallin Antibody in Western Blot (WB)

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产品信息

MA5-35646

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

ARC1245

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 6-95 of human CRYAA (P02489)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

3 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2849546

产品详细信息

Immunogen sequence: QHPWFKRTLG PFYPSRLFDQ FFGEGLFEYD LLPFLSSTIS PYYRQSLFRT VLDSGISEVR SDRDKFVIFL DVKHFSPEDL TVKVQDDFVE

靶标信息

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.

仅用于科研。不用于诊断过程。未经明确授权不得转售。