Invitrogen

Aminoacylase Recombinant Rabbit Monoclonal Antibody (006)

Aminoacylase Antibody in Immunocytochemistry (ICC/IF)

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Aminoacylase Antibody (MA5-28996) in ICC/IF

Immunofluorescence staining of Human Aminoacylase in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Aminoacylase Recombinant Rabbit Monoclonal Antibody (6) (Product # MA5-28996, 1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser conf... View More

Aminoacylase Antibody in Flow Cytometry (Flow)

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产品信息

MA5-28996

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:20-1:100

流式细胞分析 (Flow)

1:25-1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

006

抗原

Recombinant Human ACY1/Aminoacylase-1 protein (Met1-Ser408)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2784936

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Human ACY1/Aminoacylase-1.

靶标信息

ACY1 encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21. 1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene. A related pseudogene has been identified on chromosome 18.

仅用于科研。不用于诊断过程。未经明确授权不得转售。