Invitrogen

Amphiregulin Polyclonal Antibody

Amphiregulin Antibody in Western Blot (WB)

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Amphiregulin Antibody (PA5-16621) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with PMA (100 ng PMA for 16h) (Lane 2), Caco-2 (Lane 3), A-431 (Lane 4), PC-3 (Lane 5), U-2 OS (Lane 6) and HCT-116 (Lane7).The blots were probed with Amphiregulin Rabbit polyclonal Antibody (Product # PA5-16621, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 25 kDa band corresponding t... View More

Amphiregulin Antibody in Immunoprecipitation (IP)

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产品信息

PA5-16621

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

免疫沉淀 (IP)

10 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic 19-mer peptide, corresponding to aa 26-44 from the secreted amphiregulin of human origin

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_10981232

产品详细信息

PA5-16621 targets Amphiregulin in IP and WB applications and shows reactivity with Human and mouse samples.

The PA5-16621 immunogen is a synthetic 19-mer peptide, corresponding to aa 26-44 from the secreted amphiregulin of human origin.

靶标信息

Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.

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