Invitrogen

Amphiregulin Monoclonal Antibody (AREG559), eBioscience™

Amphiregulin Antibody in Western Blot (WB)

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Amphiregulin Antibody (14-5370-82) in WB

Western Blot analysis was performed on whole cell extracts of human peripheral blood cells treated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975) overnight (Lane 1) or only with Protein Transport Inhibitor Cocktail (Product # 00-4980) (Lane 2). The blot was blocked with 5% non-fat milk followed by probing with 5 µg/mL of Amphiregulin Monoclonal Antibody (Product # 14-5370-82) . Bands were visualized by chemiluminescence using Rat anti-Mouse IgG Secondary Antibody, HRP followed by SuperSignal™ West Pi... View More

Amphiregulin Antibody in Flow Cytometry (Flow)

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产品信息

14-5370-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-10 µg/mL

流式细胞分析 (Flow)

Assay-Dependent

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产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

AREG559

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_2716935

产品详细信息

The AREG858 monoclonal antibody reacts with human Amphiregulin (AR). AR is a member of the Epidermal Growth Factor (EGF) family and is expressed as a transmembrane precursor protein and released by proteolytic cleavage. Soluble AR is a ligand for the EGF receptor (EGFR) and the interaction promotes growth of normal epithelial cells, fibroblasts and keratinocytes. In the context of the immune system AR functions as an effector molecule for tissue repair and homeostasis, mediates resistance and tolerance to infection, and suppresses inflammation. Under different disease and activating conditions AR is expressed in T cells, group 2 innate lymphoid cells, basophils, mast cells, eosinophils, and neutrophils.

Applications Reported: This AREG559 antibody has been reported for use in flow cytometric analysis and western blot.

Applications Tested: This AREG559 antibody has been tested by western blot analysis of stimulated normal human peripheral blood cells. This can be used 1-10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.

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