Invitrogen

Amyloid Fibrils Polyclonal Antibody

Amyloid Fibrils Antibody in Western Blot (WB)

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Amyloid Fibrils Antibody (PA5-77843) in WB

Western blot analysis of Amyloid Fibrils in human Abeta42 fibrils and prefibrillar oligomers. The sample was incubated with Amyloid Fibrils polyclonal antibody (Product # PA5-77843) using a dilution of 1:1000.

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产品信息

PA5-77843

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:100

免疫细胞化学 (ICC/IF)

Assay-Dependent

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酶联免疫吸附实验 (ELISA)

Assay-Dependent

免疫沉淀 (IP)

Assay-Dependent

点杂交 (DB)

1:1,000

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Fibrils prepared from human amyloid beta 42 peptide

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2735453

产品详细信息

Specificity: recognizes generic epitopes common to many amyloid fibrils and fibrillar oligomers, but not prefibrilllar oligomers or natively folded proteins. Expected to detect Mouse and Rat based on species homology.

A 1:1,000 dilution of PA5-77843 was sufficient for detection of amyloid fibrils on PVDF membranes using transferred fibrils by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.|Recognizes generic epitopes common to many amyloid fibrils and fibrillar oligomers, but not prefibrillar oligomers or natively folded proteins. Expected to detect in Mouse and Rat based on species homology.

靶标信息

Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres. These include the amyloid-beta peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer's symptomology, the deposition of alpha-synuclein in the Lewy bodies of Parkinson's disease, and accumulation of polyglutamine-containing aggregates in Huntington's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A Feedback Loop between TGF-β1 and ATG5 Mediated by miR-122-5p Regulates Fibrosis and EMT in Human Trabecular Meshwork Cells.

Current issues in molecular biology

Chakraborthy M,Rao A

PA5-77843 was used in Immunocytochemistry-immunofluorescence to show that TGF-β1 induces autophagy in primary HTM cells and a positive feedback loop exists between TGF-β1 and ATG5 that regulated TGF downstream effects mainly mediated by Smad3 signaling with miR-122-5p also playing a role.

Mon Mar 13 00:00:00 UTC 2023

Primary Human Trabecular Meshwork Model for Pseudoexfoliation.

Cells

Chakraborty M,Sahay P,Rao A

PA5-77843 was used in Immunocytochemistry-immunofluorescence to create a workable in vitro model of pseudoexfoliation syndrome (PXF) using primary human TM (HTM) cell lines simulating human disease.

Tue Dec 07 00:00:00 UTC 2021

生物信息学

蛋白别名: A11; ABPP; AD; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; amyloid beta (A4) precursor protein; Amyloid precursor protein; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Amyloid-beta precursor protein; APP; APPI; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; Cerebral vascular amyloid peptide; CVAP; Fibrils; OC; peptidase nexin-II; PN-II; PreA4; Protease nexin-II; testicular tissue protein Li 2

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基因别名: A4; AAA; ABETA; ABPP; AD1; APP; APPI; CTFgamma; CVAP; PN-II; PN2

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UniProt ID: (Human) P05067

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Entrez Gene ID: (Human) 351

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