Invitrogen

Angiostatin Polyclonal Antibody

Angiostatin Antibody in Immunocytochemistry (ICC/IF)

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Angiostatin Antibody (PA1-600) in ICC/IF

Immunofluorescent analysis of Angiostatin (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Angiostatin polyclonal antibody (Product # PA1-600) in 3% BS... View More

Angiostatin Antibody in Western Blot (WB)

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产品信息

PA1-600

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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免疫细胞化学 (ICC/IF)

1:10-1:100

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: V(98) Y L S E C K T G I G N G Y R G T M S(116)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2167241

产品详细信息

PA1-600 detects recombinant human and mouse angiostatin.

PA1-600 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects a 50 kDa protein representing recombinant human angiostatin.

PA1-600 immunizing peptide corresponds to amino acid residues 98-116 from mouse angiostatin. This sequence is 95% and 84% conserved in rat and human angiostatin, respectively.

PA1-600 can be used with blocking peptide PEP-111.

靶标信息

The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Janus Kinase 1 Plays a Critical Role in Mammary Cancer Progression.

Cell reports

Wehde BL,Rädler PD,Shrestha H,Johnson SJ,Triplett AA,Wagner KU

PA1-600 was used in Western Blotting to determine the role of Janus kinases in IL-6 cytokine signalling in metastatic cancer.

Tue Nov 20 00:00:00 UTC 2018

Treatment of medulloblastoma with oncolytic measles viruses expressing the angiogenesis inhibitors endostatin and angiostatin.

BMC cancer

Hutzen B,Bid HK,Houghton PJ,Pierson CR,Powell K,Bratasz A,Raffel C,Studebaker AW

PA1-600 was used in western blot to study the use of oncolytic measles viruses expressing endostatin and angiostatin for medulloblastoma therapy

Wed Mar 19 00:00:00 UTC 2014

Treatment of medulloblastoma with oncolytic measles viruses expressing the angiogenesis inhibitors endostatin and angiostatin.

BMC cancer

Hutzen B,Bid HK,Houghton PJ,Pierson CR,Powell K,Bratasz A,Raffel C,Studebaker AW

PA1-600 was used in western blot to study the use of oncolytic measles viruses expressing endostatin and angiostatin for medulloblastoma therapy

Wed Mar 19 00:00:00 UTC 2014

生物信息学

蛋白别名: Activation peptide; Angiostatin; Microplasmin; plasmin; Plasmin heavy chain A; Plasmin heavy chain A, short form; Plasmin light chain B; Plasminogen

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基因别名: AI649309; Pg; PLG

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UniProt ID: (Human) P00747, (Mouse) P20918

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Entrez Gene ID: (Human) 5340, (Mouse) 18815

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功能

serine-type endopeptidase activity receptor binding protein binding serine-type peptidase activity protein domain specific binding apolipoprotein binding endopeptidase activity peptidase activity hydrolase activity

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