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Angiostatin Antibody (PA1-600) in ICC/IF
Immunofluorescent analysis of Angiostatin (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Angiostatin polyclonal antibody (Product # PA1-600) in 3% BS... View More
Product Details
PA1-600
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: V(98) Y L S E C K T G I G N G Y R G T M S(116)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2167241
Product Specific Information
PA1-600 detects recombinant human and mouse angiostatin.
PA1-600 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects a 50 kDa protein representing recombinant human angiostatin.
PA1-600 immunizing peptide corresponds to amino acid residues 98-116 from mouse angiostatin. This sequence is 95% and 84% conserved in rat and human angiostatin, respectively.
PA1-600 can be used with blocking peptide PEP-111.
Target Information
The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Activation peptide; Angiostatin; Microplasmin; plasmin; Plasmin heavy chain A; Plasmin heavy chain A, short form; Plasmin light chain B; Plasminogen
Gene Aliases: AI649309; Pg; PLG
UniProt ID: (Human) P00747, (Mouse) P20918
Entrez Gene ID: (Human) 5340, (Mouse) 18815
proteolysis
blood coagulation
hemostasis
negative regulation of cell-substrate adhesion
negative regulation of angiogenesis
extracellular matrix disassembly
tissue regeneration
fibrinolysis
myoblast differentiation
muscle cell cellular homeostasis
tissue remodeling
proteolysis involved in cellular protein catabolic process
negative regulation of fibrinolysis
positive regulation of fibrinolysis
trophoblast giant cell differentiation
labyrinthine layer blood vessel development
mononuclear cell migration
platelet degranulation
negative regulation of cell proliferation
cellular protein metabolic process
negative regulation of cell-cell adhesion mediated by cadherin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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