Invitrogen

Annexin A2 Monoclonal Antibody (Z014)

Annexin A2 Antibody in Immunocytochemistry (ICC/IF)

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Annexin A2 Antibody (03-4400) in ICC/IF

Immunofluorescence analysis of Annexin A2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 15 minutes at room temperature. The cells were labeled with Annexin A2 Monoclonal Antibody (Z014) (Product # 03-4400) at 5 µg in 0.1% BSA, incubated at 4 degree celsi... View More

Annexin A2 Antibody in Western Blot (WB)

Annexin A2 Antibody in Neutralization (Neu)

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产品信息

03-4400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-1.0 µg/mL

查看7篇已发表文章 7篇已发表文章

免疫组化 (IHC)

5-10 µg/mL

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

0.1-1.0 µg/mL

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

中和实验 (Neu)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Bovine, Dog, Human, Sheep

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

Z014

抗原

Annexin II purified from bovine lung.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

purified

保存液

proprietary buffer

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2532925

靶标信息

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

METTL3-mediated ANXA2 inhibition confers neuroprotection in ischemic stroke: Evidence from in vivo and in vitro studies.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liu B,Xing Z,Song F,Li D,Zhao B,Xu C,Xia W,Ji H

03-4400 was used in Flow Cytometry to reveal the neuroprotective effects of METTL3-mediated ANXA2/p38MAPK/MMP-9 inhibition against ischemic stroke.

Sat Jul 01 00:00:00 UTC 2023

Annexin A2 Enhances Complement Activation by Inhibiting Factor H.

Journal of immunology (Baltimore, Md. : 1950)

Renner B,Tong HH,Laskowski J,Jonscher K,Goetz L,Woolaver R,Hannan J,Li YX,Hourcade D,Pickering MC,Holers VM,Thurman JM

34400 was used in flow cytometry and immunohistochemistry - frozen section to identify annexin A2 as a binding partner of factor H

Mon Feb 01 00:00:00 UTC 2016

Up-regulation of annexin A2 in cholangiocarcinoma caused by Opisthorchis viverrini and its implication as a prognostic marker.

International journal for parasitology

Yonglitthipagon P,Pairojkul C,Chamgramol Y,Mulvenna J,Sripa B

03-4400 was used in Western Blotting to study how ANXA2 expression in tumors may be useful for predicting the poor outcome of CCA patients.

Sun Aug 15 00:00:00 UTC 2010

Up-regulation of annexin A2 in cholangiocarcinoma caused by Opisthorchis viverrini and its implication as a prognostic marker.

International journal for parasitology

Yonglitthipagon P,Pairojkul C,Chamgramol Y,Mulvenna J,Sripa B

03-4400 was used in Western Blotting to study how ANXA2 expression in tumors may be useful for predicting the poor outcome of CCA patients.

Sun Aug 15 00:00:00 UTC 2010

Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells.

The Journal of biological chemistry

Hastie C,Masters JR,Moss SE,Naaby-Hansen S

03-4400 was used in blocking or activating experiment to examine the effect of interferon-gamma treatment on cell surface protein expression using prostate cancer cells

Fri May 02 00:00:00 UTC 2008

Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells.

The Journal of biological chemistry

Hastie C,Masters JR,Moss SE,Naaby-Hansen S

03-4400 was used in blocking or activating experiment to examine the effect of interferon-gamma treatment on cell surface protein expression using prostate cancer cells

Fri May 02 00:00:00 UTC 2008

Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells.

The Journal of biological chemistry

Hastie C,Masters JR,Moss SE,Naaby-Hansen S

03-4400 was used in blocking or activating experiment to examine the effect of interferon-gamma treatment on cell surface protein expression using prostate cancer cells

Fri May 02 00:00:00 UTC 2008

Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells.

The Journal of biological chemistry

Hastie C,Masters JR,Moss SE,Naaby-Hansen S

03-4400 was used in blocking or activating experiment to examine the effect of interferon-gamma treatment on cell surface protein expression using prostate cancer cells

Fri May 02 00:00:00 UTC 2008

Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells.

The Journal of biological chemistry

Hastie C,Masters JR,Moss SE,Naaby-Hansen S

03-4400 was used in blocking or activating experiment to examine the effect of interferon-gamma treatment on cell surface protein expression using prostate cancer cells

Fri May 02 00:00:00 UTC 2008

Involvement of annexin A2 in anti-beta2GPI/beta2GPI-induced tissue factor expression on monocytes.

Cell research

Zhou H,Ling S,Yu Y,Wang T,Hu H

03-4400 was used in Western Blotting to show that annexin 2 is expressed on monocytes, and is involved in monocyte activation and tissue factor induced by anti-β2GPI/β2GPI.

Wed Aug 01 00:00:00 UTC 2007

Annexin II mediates plasminogen-dependent matrix invasion by human monocytes: enhanced expression by macrophages.

Blood

Brownstein C,Deora AB,Jacovina AT,Weintraub R,Gertler M,Khan KM,Falcone DJ,Hajjar KA

Thu Jan 01 00:00:00 UTC 2004

An annexin 2 phosphorylation switch mediates p11-dependent translocation of annexin 2 to the cell surface.

The Journal of biological chemistry

Deora AB,Kreitzer G,Jacovina AT,Hajjar KA

Fri Oct 15 00:00:00 UTC 2004

An annexin 2 phosphorylation switch mediates p11-dependent translocation of annexin 2 to the cell surface.

The Journal of biological chemistry

Deora AB,Kreitzer G,Jacovina AT,Hajjar KA

Fri Oct 15 00:00:00 UTC 2004

Integrin alphaMbeta2 orchestrates and accelerates plasminogen activation and fibrinolysis by neutrophils.

The Journal of biological chemistry

Pluskota E,Soloviev DA,Bdeir K,Cines DB,Plow EF

03-4400 was used in blocking or activating experiment to show that the alpha(M)beta(2) regulates neutrophil-dependent fibrinolysis

Fri Apr 23 00:00:00 UTC 2004

Inactivation of annexin II tetramer by S-nitrosoglutathione.

European journal of biochemistry

Liu L,Enright E,Sun P,Tsai SY,Mehta P,Beckman DL,Terrian DM

Sun Sep 01 00:00:00 UTC 2002

Inactivation of annexin II tetramer by S-nitrosoglutathione.

European journal of biochemistry

Liu L,Enright E,Sun P,Tsai SY,Mehta P,Beckman DL,Terrian DM

Sun Sep 01 00:00:00 UTC 2002

Suppressive effect on lymphoproliferation in vitro by soluble annexin II released from isolated placental membranes.

American journal of reproductive immunology (New York, N.Y. : 1989)

Aarli A,Kristoffersen EK,Jensen TS,Ulvestad E,Matre R

03-4400 was used in western blot to test if annexin II is released from syncytiotrophoblast microvillous plasma membranes and if it has any immunosuppressive effect.

Sat Nov 01 00:00:00 UTC 1997

生物信息学

蛋白别名: annexin 2; Annexin A2; Annexin II; Annexin-2; Calpactin I heavy chain; calpactin I heavy chain (p36); calpactin I heavy polypeptide; Calpactin-1 heavy chain; chromobindin 8; Chromobindin-8; epididymis secretory protein Li 270; Lipocortin II; p36; PAP-IV; Placental anticoagulant protein IV; Protein I

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基因别名: ANX2; ANX2L4; ANXA2; CAL1H; HEL-S-270; LIP2; LPC2; LPC2D; P36; PAP-IV