Invitrogen

Annexin V Polyclonal Antibody

2 Published Figures

7 References

View all (31) Annexin V antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
Published Figures (2)

FIGURE: 1 / 8

Annexin V Antibody (PA5-27872) in WB

Western blot was performed using Anti- Annexin V Polyclonal Antibody (Product # PA5-27872) and a 35 kDa band corresponding to Annexin V was observed across the cell lines tested. Whole cell extracts (30 µg) of HeLa (Lane 1), MCF-7 (Lane 2), MDA-MB231 (Lane 3), Hep G2 (Lane 4), tissues extracts of Mouse Liver( Lane 5), Mouse Skeletal Muscle (Lane 6) and Rat Skeletal Muscle (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Prod... View More

Annexin V Antibody in Flow Cytometry (Flow)

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Product Details

PA5-27872

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:50,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 3 publications 3 publications

Flow Cytometry (Flow)

1:50-1:200

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 320 of Human Annexin V

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2545348

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, GL261, C8D30, NIH-3T3, Raw 264.7, PC-12, Rat2.

Predicted reactivity: Mouse (93%), Rat (91%), Dog (98%), Chimpanzee (100%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Intercellular Adhesion Molecule-1-Induced Posttraumatic Brain Injury Neuropathology in the Prefrontal Cortex and Hippocampus Leads to Sensorimotor Function Deficits and Psychological Stress.

eNeuro

Bhowmick S,Malat A,Caruso D,Ponery N,D'Mello V,Finn C,Abdul-Muneer PM

Published figure using Annexin V polyclonal antibody (Product # PA5-27872) in Western Blot

Fri Aug 13 00:00:00 UTC 2021

Tissue Architectural Cues Drive Organ Targeting of Tumor Cells in Zebrafish.

Cell systems

Paul CD,Bishop K,Devine A,Paine EL,Staunton JR,Thomas SM,Thomas JR,Doyle AD,Miller Jenkins LM,Morgan NY,Sood R,Tanner K

PA5-27872 was used in Immunocytochemistry-wholemount to examine the role of physical factors in non-random organ colonization using a zebrafish xenograft model.

Wed Aug 28 00:00:00 UTC 2019

Deficiency of mouse mast cell protease 4 mitigates cardiac dysfunctions in mice after myocardium infarction.

Biochimica et biophysica acta. Molecular basis of disease

Wang Y,Liu CL,Fang W,Zhang X,Yang C,Li J,Liu J,Sukhova GK,Gurish MF,Libby P,Shi GP,Zhang J

PA5-27872 was used in Flow cytometry/Cell sorting to study the role of mouse mast cell protease-4 in cardiac dysfunction post-myocardial infarction.

Sat Jun 01 00:00:00 UTC 2019

Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury.

Brain sciences

Bhowmick S,D'Mello V,Ponery N,Abdul-Muneer PM

Published figure using Annexin V polyclonal antibody (Product # PA5-27872) in Western Blot

Sat Jan 06 00:00:00 UTC 2018

Shielding Engineered Islets With Mesenchymal Stem Cells Enhance Survival Under Hypoxia.

Journal of cellular biochemistry

Chandravanshi B,Bhonde RR

PA5-27872 was used in western blot to develop methods to improve islet survival in hypoxia

Fri Sep 01 00:00:00 UTC 2017

Stiffened Extracellular Matrix and Signaling from Stromal Fibroblasts via Osteoprotegerin Regulate Tumor Cell Invasion in a 3-D Tumor in Situ Model.

Cancer microenvironment : official journal of the International Cancer Microenvironment Society

McLane JS,Ligon LA

PA527872 was used in immunocytochemistry to ask if alterations in stromal fibroblast signaling or stiffness regulate tumor cell invasion

Thu Dec 01 00:00:00 UTC 2016

Palladin mediates stiffness-induced fibroblast activation in the tumor microenvironment.

Biophysical journal

McLane JS,Ligon LA

PA5-27872 was used in Immunocytochemistry to identify the actin-associated protein palladin as a key node in these signaling pathways that result in fibroblast activation.

Tue Jul 21 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Anchorin CII; annexin 5; Annexin A5; Annexin V; Annexin-5; Calphobindin I; CBP-I; CPB-I; Endonexin II; epididymis secretory protein Li 7; Lipocortin V; PAP-I; Placental anticoagulant protein 4; Placental anticoagulant protein I; PP4; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alpha

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Gene Aliases: ANX5; ANXA5; ENX2; HEL-S-7; LC5; PP4; R74653; RPRGL3

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UniProt ID: (Human) P08758, (Mouse) P48036, (Rat) P14668

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Entrez Gene ID: (Human) 308, (Mouse) 11747, (Rat) 25673

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Function(s)

phospholipase inhibitor activity calcium-transporting ATPase activity calcium ion binding protein binding phospholipid binding calcium-dependent phospholipid binding peptide hormone binding receptor tyrosine kinase binding calcium-binding protein

Process(es)

positive regulation of defense response to virus by host signal transduction blood coagulation negative regulation of blood coagulation positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of catalytic activity protein homooligomerization response to calcium ion calcium ion transmembrane transport cellular response to lead ion response to thyroid hormone cellular response to gonadotropin-releasing hormone mitophagy in response to mitochondrial depolarization xenophagy regulation of sperm motility negative regulation of prolactin secretion hemostasis response to organic substance negative regulation of coagulation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Annexin V Polyclonal Antibody

Annexin V Antibody (PA5-27872) in WB

Product Details

PA5-27872

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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