Invitrogen

Apelin Polyclonal Antibody

Apelin Antibody in Immunocytochemistry (ICC/IF)

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Apelin Antibody (PA5-114860) in ICC/IF

Immunocytochemistry analysis of Apelin in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114860) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

Apelin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-114860

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

in situ PLA (PLA)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Pig

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human APLN(Accession Q9ULZ1), corresponding to amino acid residues E35-F77.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2899496

产品详细信息

Antibody detects endogenous levels of total Apelin.

靶标信息

This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The cardioprotective effects of secoisolariciresinol diglucoside (flaxseed lignan) against cafeteria diet-induced cardiac fibrosis and vascular injury in rats: an insight into apelin/AMPK/FOXO3a signaling pathways.

Frontiers in pharmacology

Abdelwahab AH,Negm AM,Mahmoud ES,Salama RM,Schaalan MF,El-Sheikh AAK,Ramadan BK

Published figure using Apelin polyclonal antibody (Product # PA5-114860) in Western Blot

Fri Jul 28 00:00:00 UTC 2023

Apelin is expressed in intimal smooth muscle cells and promotes their phenotypic transition.

Scientific reports

Cardoso Dos Santos LM,Azar P,Brun C,König S,Roatti A,Baertschi AJ,Chaabane C,Bochaton-Piallat ML

PA5-114860 was used in Proximity Ligation Assay (PLA) to suggest that apelin acts via nuclear re-localization of S100A4, raising the possibility of a new pro-atherogenic relationship between apelin and S100A4.