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ApoA1 Antibody (MA5-14667) in IHC (P)
Immunohistochemistry was performed on human liver tissue sections. To expose Apo A-1, tissues were microwaved for 10 minutes in 10mM sodium citrate, pH 6.0. Following antigen retrieval, endogenous peroxidases were blocked in a 3% hydrogen peroxide-methanol solution for 15 minutes, and then tissues were blocked in 3% BSA in PBS for 30 minutes at room temperature. Tissues were probed with an Apo A-1 monoclona... View More
产品信息
MA5-14667
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
6001
抗原
Purified human plasma Apo A-1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Ion-exchange chromatography
保存液
PBS, pH 7.4, with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10980499
产品详细信息
By sandwich ELISA, MA5-14667 can be used as a detection antibody with Product # 710263 as the coating antibody, to generate a matched pair. Using this matched pair, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. It is not recommended to use Product # 701239 for coating when MA5-14667 is used for detection. By sandwich ELISA, MA5-14667 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MA5-14667, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.
By Western blot, MA5-14667 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MA5-14667 may not be as successful for detecting endogenous Apo A-1 by Western blot.
Product MA514667 is a smaller package size of MIA1401 (formerly sold as a Seradyn product).
靶标信息
Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 1-Sep; 2-Sep; Apo a; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; Apolipoprotein A-I; Apolipoprotein A-I(1-242); Apolipoprotein A1; Apolipoprotein AI; MGC102525; MGC117399; MGC124929; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7
基因别名: apo(a); APOA1
UniProt ID: (Human) P02647
Entrez Gene ID: (Human) 335
beta-amyloid binding
protein binding
phospholipid binding
phospholipid transporter activity
high-density lipoprotein particle binding
cholesterol binding
cholesterol transporter activity
enzyme binding
phosphatidylcholine binding
apolipoprotein receptor binding
apolipoprotein A-I receptor binding
identical protein binding
chemorepellent activity
lipase inhibitor activity
phosphatidylcholine-sterol O-acyltransferase activator activity
high-density lipoprotein particle receptor binding
retinoid metabolic process
regulation of protein phosphorylation
endothelial cell proliferation
platelet degranulation
negative regulation of cytokine secretion involved in immune response
phospholipid metabolic process
phosphatidylcholine biosynthetic process
cholesterol biosynthetic process
receptor-mediated endocytosis
transforming growth factor beta receptor signaling pathway
G-protein coupled receptor signaling pathway
integrin-mediated signaling pathway
response to nutrient
cholesterol metabolic process
glucocorticoid metabolic process
negative regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of cholesterol esterification
positive regulation of triglyceride catabolic process
negative regulation of very-low-density lipoprotein particle remodeling
peripheral nervous system axon regeneration
protein oxidation
peptidyl-methionine modification
triglyceride catabolic process
lipid storage
regulation of intestinal cholesterol absorption
cholesterol transport
adrenal gland development
organ regeneration
neuron projection regeneration
regulation of Cdc42 protein signal transduction
cholesterol efflux
phospholipid efflux
negative regulation of heterotypic cell-cell adhesion
high-density lipoprotein particle remodeling
high-density lipoprotein particle assembly
high-density lipoprotein particle clearance
positive regulation of Rho protein signal transduction
lipoprotein metabolic process
lipoprotein biosynthetic process
response to drug
cholesterol homeostasis
blood vessel endothelial cell migration
response to estrogen
reverse cholesterol transport
cellular protein metabolic process
positive regulation of fatty acid biosynthetic process
negative regulation of interleukin-1 beta secretion
negative regulation of inflammatory response
protein stabilization
negative chemotaxis
positive regulation of lipoprotein lipase activity
vitamin transport
positive regulation of hydrolase activity
positive regulation of stress fiber assembly
transmembrane transport
phospholipid homeostasis
negative regulation of lipase activity
negative regulation of cell adhesion molecule production
negative regulation of response to cytokine stimulus
triglyceride homeostasis
ERK1 and ERK2 cascade
cholesterol import
positive regulation of substrate adhesion-dependent cell spreading
