Invitrogen

ApoA1 Monoclonal Antibody (6001)

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产品信息

MIA1401

应用
建议稀释比
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免疫印迹 (WB)

1:500-1:2,000
-

免疫组化（石蜡） (IHC (P))

1:100
-

酶联免疫吸附实验 (ELISA)

1 µg/mL
-

免疫沉淀 (IP)

Assay-dependent
-

放射免疫测定 (RIA)

Assay-dependent
-
产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

6001

抗原

Purified human plasma Apo A-1
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11152877

产品详细信息

By sandwich ELISA, MIA1401 can be used as a detection antibody with Product # 710263 as the coating antibody, to generate a matched pair. Using this matched pair, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. It is not recommended to use Product # 701239 for coating when MIA1401 is used for detection.MIA1401 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MIA1401, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.

By Western blot, MIA1401 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MIA1401 may not be as successful for detecting endogenous Apo A-1 by Western blot.

MIA1401 was formerly sold as a Seradyn product.

靶标信息

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 1-Sep; 2-Sep; Apo a; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; apolipoprotein A-1; Apolipoprotein A-I; Apolipoprotein A-I(1-242); Apolipoprotein A1; Apolipoprotein AI; MGC102525; MGC117399; MGC124929; preproapolipoprotein A-I; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7

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基因别名: Alp-1; apo(a); apo-AI; Apoa-1; apoA-I; APOA1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2; Sep2

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UniProt ID: (Human) P02647, (Mouse) Q00623, (Rat) P04639

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Entrez Gene ID: (Human) 335, (Mouse) 11806, (Rat) 25081

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功能
beta-amyloid binding protein binding phospholipid binding phospholipid transporter activity high-density lipoprotein particle binding cholesterol binding cholesterol transporter activity enzyme binding phosphatidylcholine binding apolipoprotein receptor binding apolipoprotein A-I receptor binding identical protein binding chemorepellent activity lipase inhibitor activity phosphatidylcholine-sterol O-acyltransferase activator activity high-density lipoprotein particle receptor binding lipid transporter activity lipid binding lipoprotein particle binding
参与通路
regulation of protein phosphorylation endothelial cell proliferation negative regulation of cytokine secretion involved in immune response lipid metabolic process phospholipid metabolic process phosphatidylcholine biosynthetic process cholesterol biosynthetic process transport lipid transport G-protein coupled receptor signaling pathway integrin-mediated signaling pathway protein localization steroid metabolic process cholesterol metabolic process glucocorticoid metabolic process negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of cholesterol esterification positive regulation of triglyceride catabolic process negative regulation of very-low-density lipoprotein particle remodeling phospholipid transport protein oxidation peptidyl-methionine modification triglyceride catabolic process lipid storage regulation of intestinal cholesterol absorption cholesterol transport adrenal gland development organ regeneration axon regeneration regulation of Cdc42 protein signal transduction cholesterol efflux phospholipid efflux negative regulation of heterotypic cell-cell adhesion very-low-density lipoprotein particle remodeling high-density lipoprotein particle remodeling high-density lipoprotein particle assembly positive regulation of Rho protein signal transduction lipoprotein metabolic process lipoprotein biosynthetic process cholesterol homeostasis blood vessel endothelial cell migration reverse cholesterol transport positive regulation of fatty acid biosynthetic process phosphatidylcholine metabolic process negative regulation of interleukin-1 beta secretion negative regulation of inflammatory response protein stabilization negative chemotaxis positive regulation of lipoprotein lipase activity vitamin transport positive regulation of hydrolase activity negative regulation of hydrolase activity positive regulation of transferase activity positive regulation of stress fiber assembly phospholipid homeostasis negative regulation of lipase activity negative regulation of cell adhesion molecule production negative regulation of response to cytokine stimulus triglyceride homeostasis cholesterol import positive regulation of substrate adhesion-dependent cell spreading transforming growth factor beta receptor signaling pathway response to nutrient peripheral nervous system axon regeneration response to drug response to estrogen ERK1 and ERK2 cascade retinoid metabolic process platelet degranulation receptor-mediated endocytosis neuron projection regeneration high-density lipoprotein particle clearance cellular protein metabolic process transmembrane transport
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