Invitrogen

ApoC3 Recombinant Rabbit Monoclonal Antibody (6H21L11)

ApoC3 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

ApoC3 Antibody (701238) in ICC/IF

Immunofluorescent analysis of ApoC3 in HepG2 cells using an ApoC3 recombinant rabbit monoclonal antibody (Product # 701238) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing subcellular cytoplasmic localization of Apo C3.

Product Image Gallery

产品信息

701238

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1-3 µg/mL

产品规格

种属反应

Human, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

6H21L11

抗原

Peptide corresponding to amino acids 37-48 of human Apolipoprotein C3

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532436

产品详细信息

This antibody is predicted to react with mouse, non-human primate and rabbit based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Apolipoprotein C-III, also known as apo-CIII, is a protein that in humans is encoded by the APOC3 gene. Apo-CIII is a component of very low density lipoprotein (VLDL). APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。