Invitrogen

Aquaporin 4 Polyclonal Antibody

Aquaporin 4 Antibody in Immunocytochemistry (ICC/IF)

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Aquaporin 4 Antibody (PA5-53234) in ICC/IF

Immunofluorescent staining of Aquaporin 4 in human cell line U-251 MG using an Aquaporin 4 Polyclonal Antibody (Product # PA5-53234) shows localization to plasma membrane.

Aquaporin 4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Aquaporin 4 Antibody in Immunohistochemistry (IHC)

Aquaporin 4 Antibody in Western Blot (WB)

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产品信息

PA5-53234

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:2,500-1:5,000

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human Aquaporin 4. Recombinant protein control fragment (Product #RP-91109).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.30 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2638104

产品详细信息

Immunogen sequence: CPDVEFKRRF KEAFSKAAQQ TKGSYMEVED NRSQVETDDL ILKPGVVHVI DVDRGEEKKG KDQSGEVLSS V

Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 92%.

靶标信息

In skeletal muscle, AQP4 (aquaporin 4 also known as mercurial insensitive water channel), localizes to the sarcolemma of fast-twitch muscle fibers. Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Remyelination in neuromyelitis optica spectrum disorder is promoted by edaravone through mTORC1 signaling activation.

Glia

Luo W,Xu H,Xu L,Jiang W,Chen C,Chang Y,Liu C,Tian Z,Qiu X,Xie C,Li X,Chen H,Lai S,Wu L,Cui Y,Tang C,Qiu W

PA5-53234 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to reveal that edaravone promotes OPC maturation and remyelination/lesion repair in NMOSD patients by activating mTORC1 signaling.

Wed Feb 01 00:00:00 UTC 2023

Astrocytes regulate vascular endothelial responses to simulated deep space radiation in a human organ-on-a-chip model.

Frontiers in immunology

Verma SD,Passerat de la Chapelle E,Malkani S,Juran CM,Boyko V,Costes SV,Cekanaviciute E

PA5-53234 was used in Immunohistochemistry and Immunocytochemistry to show that simulated deep space radiation causes vascular permeability, oxidative stress, inflammation and delayed astrocyte activation in a pattern resembling CNS responses to brain injury.

Tue Oct 25 00:00:00 UTC 2022

Astrocytes regulate vascular endothelial responses to simulated deep space radiation in a human organ-on-a-chip model.

Frontiers in immunology

Verma SD,Passerat de la Chapelle E,Malkani S,Juran CM,Boyko V,Costes SV,Cekanaviciute E

Tue Oct 25 00:00:00 UTC 2022

A Microfluidic Model of AQP4 Polarization Dynamics and Fluid Transport in the Healthy and Inflamed Human Brain: The First Step Towards Glymphatics-on-a-Chip.

Advanced biology

Soden PA,Henderson AR,Lee E

PA5-53234 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to observe AQP4 depolarization following LPS treatment, suggesting that LPS-induced drainage impairments on-chip may be driven in part by changes in AQP4-dependent fluid dynamics.

Thu Dec 01 00:00:00 UTC 2022

Engineered human blood-brain barrier microfluidic model for vascular permeability analyses.

Nature protocols

Hajal C,Offeddu GS,Shin Y,Zhang S,Morozova O,Hickman D,Knutson CG,Kamm RD

PA5-53234 was used in Immunohistochemistry (PFA fixed) to show that these properties, coupled with a brain endothelial expression profile and the capability to easily test several repeats with low reagent consumption, make this BBB model highly suitable for widespread use in academic and industrial laboratories.