Invitrogen

Arginase 1 Polyclonal Antibody

Arginase 1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 10

Arginase 1 Antibody (PA5-18392) in ICC/IF

Immunocytochemistry analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Samples were incubated with Arginase 1 polyclonal antibody (Product # PA5-18392) using a dilution of 10 µg/mL for 1 hr followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa ... View More

Arginase 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Arginase 1 Antibody in Western Blot (WB)

Arginase 1 Antibody in Flow Cytometry (Flow)

Product Image Gallery

产品信息

PA5-18392

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.01-0.03 µg/mL

- -

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

3.75 µg/mL

免疫细胞化学 (ICC/IF)

10 µg/mL

流式细胞分析 (Flow)

10 µg/mL

产品规格

种属反应

Human, Mouse, Pig, Rat

已发表种属

Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (CFGLAREGNHKPID) corresponding to the C-terminus amino acids of ARG1 (aa 303-316).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_10983554

产品详细信息

This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.

靶标信息

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Microparticles Decorated with Cell-Instructive Surface Chemistries Actively Promote Wound Healing.

Advanced materials (Deerfield Beach, Fla.)

Latif A,Fisher LE,Dundas AA,Cuzzucoli Crucitti V,Imir Z,Lawler K,Pappalardo F,Muir BW,Wildman R,Irvine DJ,Alexander MR,Ghaemmaghami AM

PA5-18392 was used in Immunohistochemistry-immunofluorescence to identify candidates which actively drive fibroblasts toward either pro- or antiproliferative functional phenotypes.

Mon Nov 28 00:00:00 UTC 2022

生物信息学

蛋白别名: A-I; AI type I arginase; Arginase; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

查看更多收起

基因别名: AI; AI256583; Arg-1; ARG1; PGIF