Invitrogen

Arp2 Polyclonal Antibody

Arp2 Antibody in Immunocytochemistry (ICC/IF)

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Arp2 Antibody (PA5-19760) in ICC/IF

Immunofluorescence analysis of Arp2 was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Arp2 Polyclonal Antibody (Product # PA5-19760) at 1µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 ... View More

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Arp2 Antibody — Antibody specificity was demonstrated by siRNA mediated KD of target protein. HeLa cells were transfected with Arp2 siRNA and reduction of signal was observed in Western Blot using Arp2 Polyclonal Antibody (Product # PA5-19760). {KD}

产品信息

PA5-19760

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

1 µg/mL

免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Human, Mouse, Xenopus

已发表种属

Xenopus

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Arp2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.8 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 10mg/mL BSA

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10980708

产品详细信息

This antibody is predicted to react with rat, chicken, cow and orangutan based on sequence homology.

靶标信息

ARP2 (actin-related protein 2) is an ATP-binding component of the ARP2/3 complex - a multiprotein complex that mediates actin polymerization upon stimulation by the nucleation-promoting factor (NPF). The ARP2/3 complex mediates the formation of branched actin networks in the cytoplasm and provides the force for cell motility. The ARP2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA.

仅用于科研。不用于诊断过程。未经明确授权不得转售。