Invitrogen

Arp3 Polyclonal Antibody

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Arp3 Antibody in Western Blot (WB)

Arp3 Antibody in Western Blot (WB)

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Arp3 Antibody (PA5-17288) in WB

Western blot was performed using Anti-Arp3 Polyclonal Antibody (Product # PA5-17288) and an ~47 kDa band was observed across the panel tested except Mouse and Rat Skeletal Muscle. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MCF-7 (Lane 2), PC-3 (Lane 3), U-87 MG (Lane 4), HeLa (Lane 5), HEL 92.1.7 (Lane 6), tissue extracts (30 µg lysate) of Mouse Thymus (Lane 7), Mouse Skeletal Muscle (Lane 8), Rat Thymus (Lane 9) and Rat Skeletal Muscle (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302B... View More

Arp3 Antibody in Western Blot (WB)

Arp3 Antibody in Western Blot (WB)

Arp3 Antibody in Western Blot (WB)

Arp3 Antibody in Immunocytochemistry (ICC/IF)

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Arp3 Antibody in Western Blot (WB) — Western blot was performed using Anti-Arp3 Polyclonal Antibody (Product # PA5-17288) and an ~47 kDa band was observed across the panel tested except Mouse and Rat Skeletal Muscle. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MCF-7 (Lane 2), PC-3 (Lane 3), U-87 MG (Lane 4), HeLa (Lane 5), HEL 92.1.7 (Lane 6), tissue extracts (30 µg lysate) of Mouse Thymus (Lane 7), Mouse Skeletal Muscle (Lane 8), Rat Thymus (Lane 9) and Rat Skeletal Muscle (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Arp3 Antibody in Western Blot (WB) — Western blot analysis of ARP3 in extracts from Jurkat and COS cells using ARP3 polyclonal antibody (Product # PA5-17288).

Arp3 Antibody in Western Blot (WB) — Knockdown of Arp3 was achieved by transfecting MCF-7 cells with Arp3 specific siRNAs (Silencer® select Product # s19640, s19641). Western blot analysis (Fig. a) was performed using whole cell extracts from the MCF-7 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Arp3 Rabbit Polyclonal Antibody (Product # PA5-17288, 1:1000 dilution) and Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Arp3.

Arp3 Antibody in Immunocytochemistry (ICC/IF) — Published figure using Arp3 polyclonal antibody (Product # PA5-17288) in Immunocytochemistry

Arp3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF-7 cells were transfected with Arp3 siRNA and a decrease in signal intensity was observed in western blot application using Anti-Arp3 Polyclonal Antibody (Product # PA5-17288). {KD}

Arp3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF-7 cells were transfected with Arp3 siRNA and a decrease in signal intensity was observed in western blot application using Anti-Arp3 Polyclonal Antibody (Product # PA5-17288). {RE}

产品信息

PA5-17288

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

-

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产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the amino terminus of human ARP3

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

66 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10981744

产品详细信息

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with endogenous levels of total ARP2 protein.

靶标信息

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The centrosome is an actin-organizing centre.

Nature cell biology

Farina F,Gaillard J,Guérin C,Couté Y,Sillibourne J,Blanchoin L,Théry M

PA517288 was used in immunocytochemistry to investigate the connection between the centrosome and actin filaments

Fri Jan 01 00:00:00 UTC 2016

Enterohemorrhagic E. coli requires N-WASP for efficient type III translocation but not for EspFU-mediated actin pedestal formation.

PLoS pathogens

Vingadassalom D,Campellone KG,Brady MJ,Skehan B,Battle SE,Robbins D,Kapoor A,Hecht G,Snapper SB,Leong JM

PA5-17288 was used in western blot to investigate the role of N-WASP in type III translocation in enterohemorrhagic Escherichia coli

Thu Aug 19 00:00:00 UTC 2010

生物信息学

蛋白别名: Actin-like protein 3; Actin-related protein 3; actin-related protein 3 homolog (yest); ARP3 actin-related protein 3 homolog

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基因别名: 1200003A09Rik; ACTR3; ARP3

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UniProt ID: (Human) P61158, (Rat) Q4V7C7, (Mouse) Q99JY9

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Entrez Gene ID: (Human) 10096, (Rat) 81732, (Mouse) 74117

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structural constituent of cytoskeleton protein binding ATP binding actin filament binding nucleotide binding actin binding actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

movement of cell or subcellular component establishment or maintenance of cell polarity asymmetric cell division meiotic chromosome movement towards spindle pole meiotic cytokinesis Arp2/3 complex-mediated actin nucleation Fc-gamma receptor signaling pathway involved in phagocytosis ephrin receptor signaling pathway spindle localization cilium morphogenesis response to carbohydrate positive regulation of lamellipodium assembly positive regulation of actin filament polymerization regulation of myosin II filament organization positive regulation of neuron differentiation response to antibiotic positive regulation of dendrite morphogenesis meiotic cell cycle positive regulation of filopodium assembly actin filament organization cell projection organization