Invitrogen

Arp3 Polyclonal Antibody

Arp3 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

Arp3 Antibody (PA5-80307) in ICC/IF

Immunofluorescence staining of Arp3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Arp3 Polyclonal Antibody (Product # PA5-80307, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Arp3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

产品信息

PA5-80307

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:300-1:10,000

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

1:5,000-1:10,000

免疫沉淀 (IP)

1-4 µL/mg of lysate

产品规格

种属反应

Human

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human ACTR3/Actin-related protein 3 Protein (Ala2-Ser418)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2787638

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human ACTR3/Actin-related protein 3.

靶标信息

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Lamin A-mediated nuclear lamina integrity is required for proper ciliogenesis.

EMBO reports

Fan JR,You LR,Wang WJ,Huang WS,Chu CT,Chi YH,Chen HC

PA5-80307 was used in Immunocytochemistry to uncover a functional link between nuclear lamina integrity and ciliogenesis and implicate the malfunction of primary cilia in the pathogenesis of laminopathy.