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Asialo GM1 Antibody (16-6507-39) in Flow
Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 PerCP-Cy5-5 (Product # 45-5941-82) (left) or Anti-Mouse CD11b APC (Product # 17-0112-82) (right) and Anti-Asialo-GM1 Functional Grade Purified.An Anti-Rabbit PE (Product # 12-4739-81) was used for detection.Total viable cells were used for analysis.
产品信息
16-6507-39
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Hamster,
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
偶联物
形式
Lyophilized
纯化类型
Ammonium sulfate precipitation
保存液
PBS
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10718540
产品详细信息
Description: This rabbit polyclonal antibody reacts with the asialo ganglio-N-tetraosylceramide (asialo-GM1), a glycolipid on human, mouse, rat, bovine and hamster cells. Glycolipids such as this sialyated version are defined by the specific transferases that modify the lipid. Glycolipids are targets of immune cell response. Auto-antibodies have also been identified. Additionally these glycolipids can act as co-receptors for pathogens. Asialo-GM1 is present on both NK cells and a subset of monocyte/macrophages from the mouse spleen. Expression is also found on rat and bovine brain, fetal liver and on fetal immature thymocytes. Expression has also been shown to increase with tumor progression. The antibody recognizes asialo-GM1 specifically and not other glycolipids, including GM1 and asialo-GM2.
This antibody has been reported to deplete NK cells in mice and hamsters in vivo and exhibits in vitro effects in a variety of species.
This polyclonal antibody has been reported for use in flow cytometric analysis, depletion of NK cells, and in vitro functional studies. Intravenous and IP injection of 20-50 µL has been shown to decrease NK1.1 populations after 3-4 days. Repeat injections may be necessary.
Applications Tested: This polyclonal antibody against asialo-GM1 has been tested by flow cytometric analysis of mouse splenocytes. Optimal staining concentration will vary depending on the lot. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Endotoxin: Less than 0.001 ng/µg antibody as determined by the LAL assay.
Storage and handling: It is recommended to add 1 mL of sterile (endotoxin free) room temperature water. After reconstitution pass through 0.22 µm filter prior to injection.
靶标信息
Asialo ganglio-N-tetraosylceramide (asialo-GM1) is a glycolipid on human, mouse, rat, bovine and hamster cells. Glycolipids such as this sialyated version are defined by the specific transferases that modify the lipid. Glycolipids are targets of immune cell response. Auto-antibodies have also been identified. Additionally these glycolipids can act as co-receptors for pathogens. Asialo-GM1 is present on both NK cells and a subset of monocyte/macrophages from the mouse spleen. Expression is also found on rat and bovine brain, fetal liver and on fetal immature thymocytes. Expression has also been shown to increase with tumor progression.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: AsialoGM1
