Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 5
Ataxin 1 Antibody (703273) in ICC/IF
For immunofluorescence analysis, HeLa cells were fixed and permeabilized with 0.1% saponin for detection of endogenous Ataxin 1 using Anti-Ataxin 1 Recombinant Rabbit Monoclonal Antibody (Product # 703273, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32731, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Ataxin-1 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Ant... View More
产品信息
703273
产品规格
种属反应
Human,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
16H8L13
抗原
Protein corresponding to human Ataxin 1 [aa562-aa693]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2866459
产品详细信息
This antibody is predicted to react with Mouse, Bovine.
靶标信息
The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 41-81 CAG repeats, compared to 6-39 in the normal allele, and is associated with spinocerebellar ataxia type 1 (SCA1). At least two transcript variants encoding the same protein have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: alternative ataxin1; Ataxin-1; OTTHUMP00000016065; spinocerebellar ataxia 1; Spinocerebellar ataxia type 1 protein; Spinocerebellar ataxia type 1 protein homolog
基因别名: ATX1; ATXN1; D6S504E; SCA1
UniProt ID: (Human) P54253, (Rat) Q63540
Entrez Gene ID: (Human) 6310, (Rat) 25049
transcription, DNA-templated
RNA processing
negative regulation of transcription, DNA-templated
nuclear export
negative regulation of transcription from RNA polymerase II promoter
biological_process
adult locomotory behavior
visual learning
negative regulation of phosphorylation
negative regulation of insulin-like growth factor receptor signaling pathway
positive regulation of transcription from RNA polymerase II promoter
lung alveolus development
excitatory postsynaptic potential
positive regulation of glial cell proliferation
