Invitrogen

Axl Monoclonal Antibody (MAXL8DS), PE-Cyanine7, eBioscience™

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Axl Antibody (25-1084-82) in Flow

Mouse bone marrow cells were cultured in the presence of GM-CSF for 7 days, Fc blocked with CD16/CD32 Antibody (Product # 14-0161-82) and normal mouse serum, and subsequently stained with CD11b Monoclonal Antibody, APC (Product # 17-0112-82) and 0.06 µg of Rat IgG2a kappa Isotype Control, PE-Cyanine7 (Product # 25-4321-82) (left) or 0.06 µg of Axl Monoclonal Antibody, PE-Cyanine7 (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

Product Image Gallery

Axl Antibody — Staining of mouse splenocytes cultured for 7 days in the presence of GM-CSF. As expected, Axl Monoclonal Antibody stains dendritic cells from WT mouse but not from Axl-/- mouse. Details: splenocytes from Axl-/- mouse (left panel) or WT mouse (right panel) were cultured for 7 days in the presence of GM-CSF. The cells were then Fc blocked, stained with CD11c (clone N418), and stained with Rat IgG2a Isotype control (blue histograms) or Axl (clone MAXL8DS) (purple histograms). Viable cells expressing CD11c were used for analysis. {KO}

产品信息

25-1084-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

MAXL8DS

抗原

C57BL/6 mouse Axl-human IgG Fc fusion protein

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2734852

产品详细信息

Description: This MAXL8DS monoclonal antibody recognizes mouse Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3. Within hematopoietic compartment the highest expression of Axl can be observed on dendritic cells and macrophages. Stimulation with TLR ligands has been shown to additionally upregulate this expression. This MAXL8DS antibody will work in flow cytometry on both native and fixed/permeabilized cells.

Applications Reported: This MAXL8DS antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MAXL8DS antibody has been tested by flow cytometric analysis of mouse bone marrow cells cultured in the presence of GM-CSF. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-34) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

靶标信息

AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

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