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B3GNT2 Antibody (PA5-144001) in ICC/IF
Immunocytochemistry analysis of B3GNT2 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in B3GNT2 Polyclonal antibody (Product # PA5-144001) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriat... View More
Product Details
PA5-144001
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human B3GNT2 recombinant protein (Position: R6-C397).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3075215
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, human HL-60 whole cell, human MOLT-4 whole cell, rat brain tissue, rat kidney tissue, mouse brain tissue, mouse kidney tissue. ICC/IF: MCF-7 cell. Flow: Hela cell, THP-1 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-N-a; B3GN-T1; Beta-1,3-galactosyltransferase 7; Beta-1,3-GalTase 7; beta-1,3-Gn-T1; beta-1,3-Gn-T2; Beta-1,3-N-acetylglucosaminyltransferase 1; beta-1,3-N-acetylglucosaminyltransferase 2; beta-1,3-N-acetylglucosaminyltransferase bGnT-1; beta-1,3-N-acetylglucosaminyltransferase bGnT-2; Beta-3-Gx-T7; beta3Gn-T1; beta3Gn-T2; BGnT-1; BGnT-2; N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2; UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7; UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 6; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
Gene Aliases: 3-Gn-T1; 3-Gn-T2; AA408337; AA409404; AW260308; B3Galt6; B3GALT7; B3GN-T2; B3GNT; B3GNT-2; B3GNT1; B3GNT2; beta-1; beta3Gn-T1; beta3Gn-T2; BETA3GNT; BGnT-1; BGnT-2; BGnT1; BGNT2
UniProt ID: (Human) Q9NY97, (Mouse) Q9Z222
Entrez Gene ID: (Human) 10678, (Mouse) 53625, (Rat) 305571
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