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B7-H3 (CD276) Antibody (MA5-47649) in ICC/IF
Immunocytochemistry analysis of B7-H3 (CD276) in aldehyde fixed human MCF-7 breast ductal carcinoma cells. The cells were labeled with mouse B7-H3 (CD276) monoclonal antibody (Product # MA5-47649). The antibody was detected using goat anti-mouse DyLight® 594.
产品信息
MA5-47649
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
M045
抗原
Clone (M045) was generated from a recombinant protein that included the extracellular region of human B7-H3 protein.
偶联物
形式
Liquid
浓度
0.25 mg/mL
纯化类型
Protein G
保存液
PBS with 1mg/mL BSA, 50% glycerol
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2942630
产品详细信息
Clone M045 mouse monoclonal antibody detects a 100-120 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human LNCaP, MCF-7, NCI-H28, A431, and MDA-MB-231 carcinomas. The antibody detects B7-H3 in membranes and cytoplasm in MCF7 cell after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.
靶标信息
CD276 is a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 4Ig-B7-H3; B7 homolog 3; B7-H3; CD276; CD276 antigen; Costimulatory molecule; sB7 H3; sB7H3; sCD276; soluble B7 H3; soluble B7H3; soluble CD276
基因别名: 4Ig-B7-H3; B7-H3; B7H3; B7RP-2; CD276; PSEC0249; UNQ309/PRO352
UniProt ID: (Human) Q5ZPR3
Entrez Gene ID: (Human) 80381
immune response
cell proliferation
positive regulation of bone mineralization
positive regulation of T cell proliferation
T cell activation
negative regulation of T cell proliferation
negative regulation of interferon-gamma biosynthetic process
positive regulation of interferon-gamma biosynthetic process
negative regulation of interleukin-2 biosynthetic process
positive regulation of osteoblast differentiation
negative regulation of inflammatory response
regulation of immune response
positive regulation of interleukin-2 secretion
