Invitrogen

B7-H3 (CD276) Chimeric Recombinant Rabbit Monoclonal Antibody (MJ18)

B7-H3 (CD276) Chimeric Antibody in Immunocytochemistry (ICC/IF)

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B7-H3 (CD276) Chimeric Antibody (MA5-48102) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of B7-H3 (CD276) in mouse splenocytes using B7-H3 (CD276) Chimeric Monoclonal Antibody (Product # MA5-48102). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with B7-H3 (CD276) Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor488 secondary antibody (1 µg/mL), showing weak membrane staining of a subset of cells. Th... View More

B7-H3 (CD276) Chimeric Antibody in ELISA (ELISA)

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产品信息

MA5-48102

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

10 µg/mL

流式细胞分析 (Flow)

Assay-dependent

酶联免疫吸附实验 (ELISA)

0.1 ng/mL

抑制试剂 (IA)

Assay-dependent

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

MJ18

抗原

This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1-242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.02% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_3074545

产品详细信息

Specificity: This antibody binds to mouse B7-H3 and does not react with other B7-family molecules.

Preparation: This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1-242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry.

靶标信息

CD276 is a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。