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B7-H4 Antibody (PA5-87879) in WB
Western blot analysis of B7-H4 in extracts of various cell lines. Samples were incubated with B7-H4 Polyclonal antibody (Product # PA5-87879) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
PA5-87879
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 100-260 of human B7-H4/VTCN1 (NP_0789022)
偶联物
形式
Liquid
浓度
2.91 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2804475
产品详细信息
Immunogen sequence: RTAVFADQVI VGNASLRLKN VQLTDAGTYK CYIITSKGKG NANLEYKTGA FSMPEVNVDY NASSETLRCE APRWFPQPTV VWASQVDQGA NFSEVSNTSF ELNSENVTMK VVSVLYNVTI NNTYSCMIEN DIAKATGDIK VTESEIKRRS HLQLLNSKAS L; Positive Samples: MCF-7, SKOV3, HeLa, 293T, B-cell, Mouse uterus, Rat thymus; Cellular Location: Cell membrane, Single-pass type I membrane protein
靶标信息
B7-H4 (B7x, VTCN1) a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7-H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7-H4 deficient mice are only minimally affected; suggesting B7-H4 is important in the fine tuning of the T-cell mediated immune response. B7-H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7-H4 is expressed primarily on the membrane of lymphoid cells, and as an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, it is involved in mediating cellular and humoral immune responses. Overexpression of B7-H4 is associated with certain malignancies, including ovarian and breast cancer, which may be a mechanism by which tumor cells suppress T cell immunity and facilitate tumor progression. Further, VTCN1 may be a useful biomarker for the early detection of ovarian cancer and Renal Cell Carcinoma (RCC).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7-H4; B7h.5; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; RP11-229A19.4; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; V-set domain containing T-cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; VTCN1; vtcn1 {ECO:0000312|EMBL:AAH95842.1}
基因别名: B7-H4; B7h.5; B7H4; B7S1; B7X; BC032925; PRO1291; RGD1311204; UNQ659/PRO1291; VCTN1; VTCN1
UniProt ID: (Human) Q7Z7D3, (Rat) Q501W4, (Mouse) Q7TSP5
Entrez Gene ID: (Human) 79679, (Rat) 295322, (Mouse) 242122
