Invitrogen

B7-H4 Monoclonal Antibody (188), PE, eBioscience™

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B7-H4 Antibody (12-5972-82) in Flow

Staining of non-transfected (left) and mouse B7-H4-transfected (right) L5178Y cells with 0.25 µg of Rat IgG2b kappa Isotype Control PE (Product # 12-4031-82) (open histogram) or 0.25 µg of Anti-Mouse B7-H4 PE (filled histogram). Total viable cells were used for analysis.

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产品信息

12-5972-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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功能检测 (Functional)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

188

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_466076

产品详细信息

Description: The 188 monoclonal antibody was generated against and reacts with mouse B7-H4 also known as B7S1, B7X. Cross reactivity of this antibody to other proteins has not been determined. B7-H4 is a newly discovered member of the B7 family reported to inhibit T cell activation, cell cycle progression and IL-2 production. The ligand for B7-H4 has not been identified yet. Simultaneous double staining of cells with two anti-mouse B7-H4 antibodies, Clone 9 and 188, suggests that epitopes recognized by these mAbs are different and/or there is no steric hindrance when antibodies are used together. 188 stains mouse B7-H4 transfected cells and not spleen cells. Exact expression pattern of B7-H4 has not been fully characterized.

Applications Reported: The 188 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 188 antibody has been tested by flow cytometric analysis of transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

B7-H4 (B7x, VTCN1) a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7-H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7-H4 deficient mice are only minimally affected; suggesting B7-H4 is important in the fine tuning of the T-cell mediated immune response. B7-H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7-H4 is expressed primarily on the membrane of lymphoid cells, and as an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, it is involved in mediating cellular and humoral immune responses. Overexpression of B7-H4 is associated with certain malignancies, including ovarian and breast cancer, which may be a mechanism by which tumor cells suppress T cell immunity and facilitate tumor progression. Further, VTCN1 may be a useful biomarker for the early detection of ovarian cancer and Renal Cell Carcinoma (RCC).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8⁺ T Cells.

Immunity

Li J,Lee Y,Li Y,Jiang Y,Lu H,Zang W,Zhao X,Liu L,Chen Y,Tan H,Yang Z,Zhang MQ,Mak TW,Ni L,Dong C

12-5972 was used in Flow cytometry/Cell sorting to demonstrate that increased B7S1 expression in myeloid cells from human hepatocellular carcinoma is correlated with CD8+ T cell dysfunction.

Tue Apr 17 00:00:00 UTC 2018

Control of PD-L1 Expression by Oncogenic Activation of the AKT-mTOR Pathway in Non-Small Cell Lung Cancer.

Cancer research

Lastwika KJ,Wilson W,Li QK,Norris J,Xu H,Ghazarian SR,Kitagawa H,Kawabata S,Taube JM,Yao S,Liu LN,Gills JJ,Dennis PA

12-5972 was used in Flow cytometry/Cell sorting to suggest that oncogenic activation of the AKT-mTOR pathway promotes immune escape by driving expression of PD-L1.

Fri Jan 15 00:00:00 UTC 2016

A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1.

Cell research

Sheng H,Wang Y,Jin Y,Zhang Q,Zhang Y,Wang L,Shen B,Yin S,Liu W,Cui L,Li N

12-5972 was used in Flow cytometry/Cell sorting to investigate the role that IFNγ plays in mesenchymal stem cell mediated suppression of T cell proliferation.

Fri Aug 01 00:00:00 UTC 2008

Expression of the novel co-stimulatory molecule B7-H4 by renal tubular epithelial cells.

Kidney international

Chen Y,Yang C,Xie Z,Zou L,Ruan Z,Zhang X,Tang Y,Fei L,Jia Z,Wu Y

Fri Dec 01 00:00:00 UTC 2006

生物信息学

蛋白别名: B7 homolog 4; B7-H4; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; RP11-229A19.4; T cell costimulatory molecule B7x; V-set domain containing T-cell activation inhibitor 1; vtcn1

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基因别名: B7h4; B7s1; B7x; BC032925; Vtcn1

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UniProt ID: (Mouse) Q7TSP5

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Entrez Gene ID: (Mouse) 242122

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功能

receptor binding immunoglobulin receptor superfamily

参与通路

response to protozoan immune system process positive regulation of T cell proliferation negative regulation of T cell activation interleukin-4 secretion interferon-gamma secretion positive regulation of interleukin-2 secretion