Invitrogen

B7-H7 Monoclonal Antibody (MA57YW), PE-Cyanine7, eBioscience™

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B7-H7 Antibody (25-6537-42) in Flow

HDLM-2 cells were stained with Mouse IgG1 kappa Isotype Control, PE-Cyanine7 (Product # 25-4714-80) (blue) or B7-H7 Monoclonal Antibody, PE-Cyanine7 (purple). Total viable cells, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18), were used for analysis.

Product Image Gallery

B7-H7 Antibody — Surface staining of PBMC’s, HDLM-2, and HeLa cells. As expected based on known relative expression patterns, B7-H7 clone MA57YW stains HDLM-2 cells, but not HeLa cells or human PBMC’s. Details: Staining of human peripheral blood cells (top left) were co-stained with CD14 Monoclonal Antibody, (clone 61D3) and CD3 Monoclonal Antibody, (clone OKT3). PBMC’s were stained with Mouse IgG1 kappa Isotype Control (clone P3.6.2.8.1) (blue) or B7-H7 Monoclonal Antibody. Cells were gated on CD3 positive CD14 negative (purple top right), CD3 negative and CD14 positive (blue middle left), and CD3 negative (orange middle right). HeLa (bottom left histogram), or HDLM-2 (bottom right histogram) cells were stained with Mouse IgG1 kappa Isotype Control (blue) (clone P3.6.2.8.1) or B7-H7 Monoclonal Antibody, (purple). Total viable cells, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18), were used for analysis. {RE}

产品信息

25-6537-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

MA57YW

抗原

B7-H7 recombinant protein

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2784668

产品详细信息

Description: This MA57YW monoclonal antibody recognizes human B7-H7 on live and paraformaldehyde-fixed cells.

Applications Reported: This MA57YW antibody has been reported for use in flow cytometric analysis. This MA57YW monoclonal antibody recognizes human B7-H7 on live and paraformaldehyde-fixed cells.

Applications Tested: This MA57YW antibody has been pre-diluted and tested by flow cytometric analysis of the HDLM-2 cell line. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

靶标信息

B7-H7 (also known as HHLA2) is a membrane protein with three Ig-like domains (IgV-IgC-IgV), whereas other members of the B7 family generally have only two Ig domains (IgV-IgC). B7-H7 is somewhat more closely related to B7-H3 and B7-H4 and shares 10% to 18% amino acid identity and 23% to 33% similarity to B7 family members. B7-H7 protein is widely expressed in epithelium cells of the gallbladder, breast, kidney, colon, small intestines and placenta. In the immune system, HHLA2 protein is not constitutively expressed on human monocytes/macrophages. B7-H7 is widely expressed in cancers, especially in triple negative breast cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。