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BAF53A Antibody (711446) in ICC/IF
For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized for detection of endogenous Actl6a using Anti- Actl6a Recombinant Rabbit Superclonal™ Antibody (Product # 711446, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Actl6a protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product ... View More
产品信息
711446
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
5HCLC
抗原
Peptide corresponding to Human ACTL6A (aa 124-142)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633180
产品详细信息
This antibody is predicted to react with Monkey, Pig, Rabbit and Cat
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
BAF53A is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.
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生物信息学
蛋白别名: 53 kDa BRG1-associated factor A; actin-like 6A; actin-like 6B; Actin-like protein 6A; actin-related protein 4; Actin-related protein Baf53a; ACTL6B; ArpNbeta; BAF complex 53 kDa subunit; BAF53; BAF53A; BAF53B; BRG1-associated factor 53A; hArpN alpha; hArpN beta; INO80 complex subunit K; MGC5382; MGC5382 Arp4
基因别名: ACTL6; ACTL6A; Arp4; ARPN-BETA; BAF53; BAF53A; INO80K
UniProt ID: (Human) O96019
Entrez Gene ID: (Human) 86
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
chromatin binding
transcription coactivator activity
protein binding
nucleosomal DNA binding
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
neural retina development
DNA repair
DNA recombination
chromatin remodeling
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
signal transduction
spinal cord development
regulation of growth
ATP-dependent chromatin remodeling
histone H4 acetylation
histone H2A acetylation
regulation of mitophagy
positive regulation of protein targeting to mitochondrion
