Invitrogen

BCL2L12 Recombinant Rabbit Monoclonal Antibody (JE51-66)

BCL2L12 Antibody in Immunocytochemistry (ICC/IF)

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BCL2L12 Antibody (MA5-34871) in ICC/IF

Immunofluorescent analysis of BCL2L12 in SW620 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with BCL2L12 monoclonal antibody (Product # MA5-34871) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.

BCL2L12 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

BCL2L12 Antibody in Flow Cytometry (Flow)

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产品信息

MA5-34871

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:50

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE51-66

抗原

Recombinant protein within Human BCL2L12 aa 180-320

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles, store in dark

运输条件

Wet ice

AB_2848779

产品详细信息

Positive Control: MCF-7 cell lysates, PC-3M cell lysates, A431, SW620, human lung carcinoma tissue, human gastric carcinoma tissue.

靶标信息

This gene encodes a member of a family of proteins containing a Bcl-2 homology domain 2 (BH2). The encoded protein is an anti-apoptotic factor that acts as an inhibitor of caspases 3 and 7 in the cytoplasm. In the nucleus, it binds to the p53 tumor suppressor protein, preventing its association with target genes. Overexpression of this gene has been detected in a number of different cancers. There is a pseudogene for this gene on chromosome 3. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。