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Invitrogen
BCL6 Monoclonal Antibody (BCL-DWN), PerCP-eFluor™ 710, eBioscience™
图: 1 / 1
BCL6 Antibody (46-5453-82) in Flow
Splenocytes isolated from sheep red blood cell-immunized mice were stained with Anti-Human/Mouse GL7 PE (Product # 12-5902-82) followed by intracellular staining with 0.0075 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or 0.0075 µg of Anti-Mouse Bcl-6 PerCP-eFluor® 710 (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells expressing CD11c, F4/80, Gr-1, CD4, and CD8 were excluded, while total CD45R (B220)+ cells were used for analysis.
产品信息
46-5453-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
BCL-DWN
偶联物
激发/发射光谱
482/708 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2573767
产品详细信息
Description: This BCL-DWN monoclonal antibody reacts with mouse Bcl-6, which is also known as B-cell lymphoma 6. A member of the POZ family of transcriptional repressors, Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79-kDa protein is essential for germinal center B cell proliferation and survival. Moreover, during B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt's lymphoma, and non-Hodgkin's lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1.
Applications Reported: This BCL-DWN antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This BCL-DWN antibody has been tested by flow cytometric analysis of splenocytes isolated from sheep red blood cell-immunized mice. This can be used at less than or equal to 0.015 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Bcl-6 proto-oncogene product (a Kruppel-type zinc-finger protein) is mainly expressed in normal germinal center B cells and related lymphomas. Bcl-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin's lymphomas and Bcl-6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas. Bcl-6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: B-cell leukemia/lymphoma 5; B-cell leukemia/lymphoma 6; B-cell lymphoma 5 protein (BCL5); B-cell lymphoma 6 protein homolog; cys his2 zinc finger transcription factor; LAZ-3 protein; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3); Zinc finger and BTB domain-containing protein 27 (ZBTB27); Zinc Finger Protein 51 (ZNF51)
基因别名: Bcl-6; Bcl5; Bcl6
UniProt ID: (Mouse) P41183
Entrez Gene ID: (Mouse) 12053
RNA polymerase II regulatory region sequence-specific DNA binding
intronic transcription regulatory region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
chromatin DNA binding
sequence-specific DNA binding
metal ion binding
C2H2 zinc finger transcription factor
protein import into nucleus, translocation
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
negative regulation of cell-matrix adhesion
immune system process
germinal center formation
negative regulation of type 2 immune response
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
inflammatory response
cellular response to DNA damage stimulus
Rho protein signal transduction
spermatogenesis
protein localization
negative regulation of cell proliferation
actin cytoskeleton organization
B cell differentiation
negative regulation of cell growth
positive regulation of B cell proliferation
positive regulation of histone deacetylation
negative regulation of mast cell cytokine production
negative regulation of Rho protein signal transduction
type 2 immune response
regulation of cell proliferation
positive regulation of apoptotic process
negative regulation of apoptotic process
regulation of GTPase activity
regulation of memory T cell differentiation
negative regulation of cell differentiation
negative regulation of T-helper 2 cell differentiation
positive regulation of neuron differentiation
negative regulation of Notch signaling pathway
negative regulation of transcription, DNA-templated
negative regulation of isotype switching to IgE isotypes
erythrocyte development
regulation of inflammatory response
positive regulation of cellular component movement
negative regulation of cellular senescence
