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BDNF Antibody (500-P84BT-25UG) in WB
Western Blot: To detect hBDNF by Western Blot analysis BDNF Polyclonal Antibody, Biotin (Product # 500-P84BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P84BT-25UG
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human/Murine/Rat BDNF
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930807
产品详细信息
AA Sequence of recombinant protein: MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human/Murine/Rat BDNF. Anti-Human/Murine/Rat BDNF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human BDNF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BDNF (500-P84) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BDNF.
Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P84BT-1MG will be provided as 2 x 500 µg
靶标信息
BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.
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生物信息学
蛋白别名: Abrineurin; anorexia BDNF; BDNF; brain derived neurothrophic factor; brain derived neurotrophic factor; Brain-derived neurotrophic factor; H-BDNF; neurotrophin
基因别名: ANON2; BDNF; BULN2
UniProt ID: (Human) Q9BYY7, (Rat) P23363, (Mouse) P21237
Entrez Gene ID: (Human) 627, (Rat) 24225, (Mouse) 12064
cell-cell signaling
nervous system development
axon guidance
synapse assembly
brain-derived neurotrophic factor receptor signaling pathway
positive regulation of brain-derived neurotrophic factor receptor signaling pathway
negative regulation of neuron apoptotic process
collateral sprouting
positive regulation of collateral sprouting
positive regulation of synapse assembly
ureteric bud development
behavioral fear response
response to hypoxia
chronic inflammatory response
mitochondrial electron transport, NADH to ubiquinone
transmembrane receptor protein tyrosine kinase signaling pathway
gamma-aminobutyric acid signaling pathway
negative regulation of neuroblast proliferation
axon target recognition
learning or memory
learning
circadian rhythm
feeding behavior
neuron recognition
response to light stimulus
response to light intensity
response to hormone
response to auditory stimulus
glutamate secretion
response to fluoxetine
response to activity
dendrite development
regulation of metabolic process
nerve development
neuron differentiation
regulation of axon extension
response to nutrient levels
response to food
negative regulation of synaptic transmission, GABAergic
positive regulation of peptidyl-serine phosphorylation
response to vitamin A
response to tumor necrosis factor
response to potassium ion
mechanoreceptor differentiation
response to drug
fear response
negative regulation of apoptotic process
regulation of neuron apoptotic process
positive regulation of neuron differentiation
negative regulation of striated muscle tissue development
regulation of retinal cell programmed cell death
regulation of synaptic plasticity
regulation of long-term neuronal synaptic plasticity
positive regulation of long-term neuronal synaptic plasticity
regulation of short-term neuronal synaptic plasticity
axon extension
inner ear development
cognition
positive regulation of sequence-specific DNA binding transcription factor activity
response to electrical stimulus
response to hyperoxia
excitatory postsynaptic potential
inhibitory postsynaptic potential
negative regulation of cell death
taste bud development
cellular response to tumor necrosis factor
cellular response to norepinephrine stimulus
response to anesthetic
dendrite extension
negative regulation of neuron death
response to L-glutamate
response to nerve growth factor
cellular response to nerve growth factor stimulus
neuron projection extension
positive regulation of glucocorticoid receptor signaling pathway
neuron projection morphogenesis
cellular response to acid chemical
