Invitrogen

BDNF Monoclonal Antibody (35909), APC

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BDNF Antibody (MA5-23665) in Flow

Flow cytometry of BDNF in U‚87 MG human glioblastoma/astrocytoma cell line. Samples were incubated in BDNF monoclonal antibody (Product # MA5-23665) or isotype control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer.

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产品信息

MA5-23665

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

10 µL per million cells

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

35909

抗原

Sf 21-derived recombinant human BDNF His129-Arg247

查看免疫原

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

纯化类型

Protein A/G

保存液

Buffered saline solution with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2609147

产品详细信息

In direct ELISAs, no cross-reactivity with recombinant human (rh) NT-3, rhNT-4, or rh beta -NGF is observed.

靶标信息

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

仅用于科研。不用于诊断过程。未经明确授权不得转售。