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BHLHB5 Antibody (PA5-100405) in ICC/IF
Immunocytochemistry analysis of BHLHB5 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with BHLHB5 polyclonal antibody (Product # PA5-100405) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-100405
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human BHLHE22(Accession Q8NFJ8), corresponding to amino acid residues L119-A169.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2849917
产品详细信息
Antibody detects endogenous levels of total BHLHE22.
靶标信息
BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: basic helix-loop-helix (bHLH) gene, class B, beta3; basic helix-loop-helix domain containing, class B, 5; basic helix-loop-helix domain containing, class B5; basic helix-loop-helix family, member e22; bHLHb5; bHLHe22; Class B basic helix-loop-helix protein 5; Class E basic helix-loop-helix protein 22; Protein BETA3; trinucleotide repeat containing 20; Trinucleotide repeat-containing gene 20 protein
基因别名: Beta3; BHLHB5; BHLHE22; CAGL85; TNRC20
UniProt ID: (Human) Q8NFJ8, (Mouse) Q8C6A8
Entrez Gene ID: (Human) 27319, (Mouse) 59058, (Rat) 365748
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
neurogenesis
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
corpus callosum morphogenesis
cerebral cortex regionalization
central nervous system projection neuron axonogenesis
corticospinal tract morphogenesis
anterior commissure morphogenesis
neuron differentiation
negative regulation of transcription, DNA-templated
retinal bipolar neuron differentiation
retina morphogenesis in camera-type eye
