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BID Antibody (PA5-29159) in ICC/IF
BID Polyclonal Antibody detects BID protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min. Green: BID protein stained by BID Polyclonal Antibody (Product # PA5-29159) diluted at 1:500. Blue: Hoechst 33343 staining.
产品信息
PA5-29159
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Full length human Bid Recombinant protein.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2546635
产品详细信息
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: apoptic d; apoptic death agonist; apoptotic death agonist BID; BH3-interacting domain death agonist; BID; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; desmocollin type 4; HGNC:1050; Human BID coding sequence; MGC15319; MGC42355; OTTHUMP00000196197; p22 BID
基因别名: 2700049M22Rik; AI875481; AU022477; BID; FP497
UniProt ID: (Human) P55957, (Rat) Q9JLT6, (Mouse) P70444
Entrez Gene ID: (Human) 637, (Rat) 64625, (Mouse) 12122
release of cytochrome c from mitochondria
protein targeting to mitochondrion
activation of cysteine-type endopeptidase activity involved in apoptotic process
brain development
apoptotic mitochondrial changes
response to estradiol
regulation of protein oligomerization
positive regulation of protein oligomerization
positive regulation of protein homooligomerization
glial cell apoptotic process
regulation of cell proliferation
positive regulation of apoptotic process
protein homooligomerization
establishment of protein localization to membrane
positive regulation of release of cytochrome c from mitochondria
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
mitochondrial outer membrane permeabilization
regulation of mitochondrial membrane permeability involved in apoptotic process
regulation of G1/S transition of mitotic cell cycle
positive regulation of extrinsic apoptotic signaling pathway
extrinsic apoptotic signaling pathway via death domain receptors
signal transduction in response to DNA damage
regulation of apoptotic process
neuron apoptotic process
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway
apoptotic process
