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BIN1 Antibody (PA5-116490) in ICC/IF
Immunocytochemical analysis of BIN1 in siha cells using a BIN1 polyclonal antibody (Product #PA5-116490). Formalin fixed cells, as seen in green, were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1:50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).
产品信息
PA5-116490
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein within human BIN1 aa 56-257
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2901121
产品详细信息
Positive controls: Rat spleen tissue, SiHa, rat brain tissue, human colon cancer tissue, human spleen tissue, human kidney tissue, mouse skeletal muscle tissue.
靶标信息
This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AMPH 2; Amph2; amphiphysin 2; Amphiphysin II; amphiphysin IIamph2; amphiphysin-like; Amphiphysin-like protein; box dependant MYC interacting protein 1; Box-dependent myc-interacting protein 1; Bridging integrator 1; DKFZp547F068; MGC10367; MGC105358; myc box dependent interacting protein 1; Myc box-dependent-interacting protein 1; SH3 domain-containing protein 9
基因别名: ALP-1; AMPH2; AMPHL; BIN1; BRAMP-2; SH3P9
UniProt ID: (Human) O00499, (Mouse) O08539, (Rat) O08839
Entrez Gene ID: (Human) 274, (Mouse) 30948, (Rat) 117028
endocytosis
multicellular organism development
regulation of endocytosis
cell differentiation
muscle cell differentiation
positive regulation of apoptotic process
positive regulation of GTPase activity
regulation of neuron differentiation
positive regulation of endocytosis
positive regulation of astrocyte differentiation
lipid tube assembly
regulation of cell cycle arrest
nucleus organization
cell proliferation
viral process
