Invitrogen

BMI-1 Recombinant Rabbit Monoclonal Antibody (JJ093-3)

BMI-1 Antibody in Immunocytochemistry (ICC/IF)

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BMI-1 Antibody (MA5-32486) in ICC/IF

Immunocytochemical analysis of BMI-1 in SW480 cells using a BMI-1 Monoclonal antibody (Product # MA5-32486) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

BMI-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

BMI-1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Bmi1; polycomb group ring finger 4 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-BMI-1 Recombinant Rabbit Monoclonal Antibody (JJ093-3) (Product # MA5-32486). {KD}

产品信息

MA5-32486

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

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产品规格

种属反应

Human, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JJ093-3

抗原

Synthetic peptide within Human Bmi1 aa 282-326

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809763

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Targeting SUMOylation dependency in human cancer stem cells through a unique SAE2 motif revealed by chemical genomics.

Cell chemical biology

Benoit YD,Mitchell RR,Wang W,Orlando L,Boyd AL,Tanasijevic B,Aslostovar L,Shapovalova Z,Doyle M,Bergin CJ,Vojnits K,Casado FL,Di Lu J,Porras DP,García-Rodriguez JL,Russell J,Zouggar A,Masibag AN,Caba C,Koteva K,Kinthada LK,Patel JS,Andres SN,Magolan J,Collins TJ,Wright GD,Bhatia M

MA5-32486 was used in Immunocytochemistry-immunofluorescence to uncover a unique role for SAE2 in the biology of human cancers.

Thu Oct 21 00:00:00 UTC 2021

生物信息学

蛋白别名: B lymphoma Mo-MLV insertion region 1; B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger proto-oncogene; murine leukemia viral (bmi-1) oncogene homolog; Pcgf4 antibody; Polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; Polycomb group RING finger protein 4; RING finger protein 51; RP11-573G6.1

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基因别名: BMI1; flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51

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UniProt ID: (Human) P35226

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Entrez Gene ID: (Human) 648, (Rat) 307151

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功能

protein binding zinc ion binding RING-like zinc finger domain binding promoter-specific chromatin binding chromatin binding ubiquitin-protein transferase activity sequence-specific DNA binding ubiquitin-protein transferase activator activity

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