Invitrogen

BMP-10 Monoclonal Antibody (462732)

图: 1 / 1

BMP-10 Antibody (MA5-23915) in Neu

Neutralization of BMP-10 in MC3T3‚E1 mouse preosteoblast cell line. Samples were incubated in BMP-10 monoclonal antibody (Product # MA5-23915). Recombinant Human BMP‚10 induces alkaline phosphatase production in the MC3T3‚E1 mouse preosteoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‚10 (100 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP‚10 Monoclonal Antibody. The ND50 is typically 0.15‚0.9 µg/mL.

Product Image Gallery

产品信息

MA5-23915

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

中和实验 (Neu)

0.15-0.9 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

462732

抗原

CHO-derived recombinant human BMP-10 Asn317-Arg424

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2609916

产品详细信息

In Western blots, no cross-reactivity with recombinant human (rh) BMP-1.1, 2, 3, 3b, 4, 5, 6, 7, 9, 15, or the pro region of rhBMP-10 is observed. In direct ELISAs, approximately 90% cross-reactivity with recombinant mouse BMP-10 is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

靶标信息

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-beta superfamily of structurally related signaling proteins. Members of this superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life. BMP-10 plays a crucial role in the development of the embryonic heart by acting to stimulate and maintain cardiomyocyte proliferation. It can signal through various receptor complexes usually containing BMPR-1A, BMPR-1B, ALK1, ALK3, or ALK6. The interaction of BMP-10 with its specific receptors can induce signaling initiated by the phosphorylation of SMAD transcription factors, including SMAD1, SMAD5, or SMAD8, but can also induce SMAD independent processes. BMP-10 is structurally related to BMP-9, and both can inhibit endothelial cell proliferation and migration.

仅用于科研。不用于诊断过程。未经明确授权不得转售。