Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (RR1/1), FITC, eBioscience™

CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)

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CD54 (ICAM-1) Antibody (BMS108FI) in Flow

Knockout of ICAM-1 (CD54) was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR845355_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD54 was performed by staining Raji CD54 Knock out cells (blue histogram) or Raji Cas9 control cells (pink histogram) with10 µl CD54 (ICAM-1) Monoclonal Antibody (RR1/1), FITC, eBioscience™ (Product # BMS108FI). Lossof signal was observed in the CD54 KOcells stained with CD54 antibody clone RR1/1 but not in the contr... View More

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产品信息

BMS108FI

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

10 µL/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

RR1/1

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

10 µL/Test

纯化类型

Affinity chromatography

保存液

TBS, pH 7.2-7.4, with 1% BSA

内含物

0.02% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_10597772

产品详细信息

Description: Recognizes the D1 domain of human CD54.

Intercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-betacomplex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors.

ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55 kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B cells and dendritic cells in tonsils, lymph nodes and Peyer's patches.

ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN-gamma within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR.

sICAM-1 serum levels were found elevated in acute renal graft rejection, insulin-dependent diabetis mellitus, anterior uveitis and in allergic inflammation.

Applications Tested: Flow Cytometry.

Purity: >95%.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser

靶标信息

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Azelnidipine Inhibits the Differentiation and Activation of THP-1 Macrophages through the L-Type Calcium Channel.

Journal of atherosclerosis and thrombosis

Komoda H,Shiraki A,Oyama JI,Nishikido T,Node K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # BMS108FI) in Flow Cytometry

Wed Aug 01 00:00:00 UTC 2018

Interleukin-27 enhances TNF-α-mediated activation of human coronary artery endothelial cells.

Molecular and cellular biochemistry

Qiu HN,Liu B,Liu W,Liu S

BMS108FI was used in flow cytometry to examine the effects of IL-27 and TNF-alpha on the cell surface expression of adhesion molecules, inflammatory cytokines, and chemokines

Fri Jan 01 00:00:00 UTC 2016

Identification of plumericin as a potent new inhibitor of the NF-κB pathway with anti-inflammatory activity in vitro and in vivo.

British journal of pharmacology

Fakhrudin N,Waltenberger B,Cabaravdic M,Atanasov AG,Malainer C,Schachner D,Heiss EH,Liu R,Noha SM,Grzywacz AM,Mihaly-Bison J,Awad EM,Schuster D,Breuss JM,Rollinger JM,Bochkov V,Stuppner H,Dirsch VM

BMS108FI was used in Flow cytometry/Cell sorting to identify any potential NF-κB pro-inflammatory signal inhibitors in the stem bark of Himatanthus sucuuba.

Tue Apr 01 00:00:00 UTC 2014

生物信息学

蛋白别名: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Ly 47; Major group rhinovirus receptor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells

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基因别名: BB2; CD54; ICAM1; P3.58

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UniProt ID: (Human) P05362

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Entrez Gene ID: (Human) 3383

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功能

virus receptor activity receptor activity transmembrane signaling receptor activity integrin binding protein binding

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