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产品信息
A-5770
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
已发表种属
Chemical
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Raised against the BODIPY®FL fluorophore and exhibits no appreciable cross-reaction with non-BODIPY®dyes, although it will cross react to varying extents with other BODIPY®dyes.
偶联物
形式
Liquid
浓度
3 mg/mL
纯化类型
IgG fraction
保存液
PBS, pH 7.2
内含物
5mM sodium azide
保存条件
4° C
运输条件
Wet ice
RRID
AB_2536193
产品详细信息
We offer a variety of anti-fluorescent dye antibodies that recognize specific fluorophores and, in most cases, quench their fluorescence. These anti-dye antibodies, including our antibody to the BODIPYTM FL fluorophore, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria, or other biomolecules have been internalized by endocytic or pinocytic processes.
The anti-BODIPYTM FL antibody (A-5770) was raised against the BODIPY FL fluorophore and exhibits no appreciable cross-reaction with non-BODIPY dyes, although it will cross-react to varying extents with other BODIPY dyes. In solution assays, we have found that this antibody effectively quenches the fluorescence of the BODIPY FL fluorophore, but quenches the BODIPY TR dye to a lesser extent and does not significantly quench the BODIPYTM TMR dye.
Invitrogen uses a sensitive quenching assay to ensure that this antibody is provided at a consistently high titer value. As supplied, 20 µL of the antibody solution is certified to produce ≥50% of the maximal fluorescence quenching of 1 mL of a 50 nM solution of BODIPY FL dye, assayed in 100 mM sodium phosphate, pH 8.0. Maximal quenching for BODIPY FL dye is ~80% of the fluorescence of the free dye. Due to steric hindrance, maximal fluorescence quenching of the BODIPY FL fluorophore covalently bound to protein may be significantly less. It is a good practice to centrifuge protein conjugate solutions briefly in a microcentrifuge before use; only the supernatant should then be added to the experiment. This step will eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
靶标信息
BODIPY™ FL dye is a bright, green-fluorescent dye with a high extinction coefficient and fluorescence quantum yield. In contrast to the highly water soluble fluorophores Alexa Fluor™ 488 dye and fluorescein (FITC), BODIPY FL dyes have unique hydrophobic properties ideal for staining lipids, membranes, and other lipophilic compounds.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: BodipyFL
