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产品信息
MA1-23162
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Mouse
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
8F7
抗原
Protein fragment expressed in E. coli corresponding to amino acids 341-748.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_557809
产品详细信息
Recommended positive controls: 293T.
For immunohistochemistry (paraffin), perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1M citrate buffer at 95ºC in water bath for 20 minutes). Avoid nonspecific binding by incubation in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use biotin-streptavidin amplification kit. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes.
靶标信息
BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: brca 1; brca-1; BRCA1/BRCA2-containing complex, subunit 1; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein 1; breast cancer 1; breast cancer 1 early onset; breast cancer 1, early onset; breast cancer associated 1; Breast cancer type 1 susceptibility protein; Breast cancer type 1 susceptibility protein homolog; BROVCA12; Delta772-3095; early onset breast cancer 1; Fanconi anemia, complementation group S; OTTHUMP00000212147; OTTHUMP00000212148; OTTHUMP00000212149; OTTHUMP00000212150; OTTHUMP00000212151; OTTHUMP00000212155; protein phosphatase 1, regulatory subunit 53; RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1; truncated BRCA1; truncated breast and ovarian cancer susceptibility protein 1; truncated breast and ovarian cancer sususceptibility protein 1; truncated breast cancer type 1 susceptibility protein
基因别名: BRCA1; BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53
UniProt ID: (Human) P38398, (Mouse) P48754
Entrez Gene ID: (Human) 672, (Mouse) 12189
DNA binding
chromatin binding
damaged DNA binding
transcription coactivator activity
RNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
tubulin binding
ligase activity
enzyme binding
ubiquitin protein ligase binding
transcription regulatory region DNA binding
androgen receptor binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein modifying enzyme
double-strand break repair via homologous recombination
DNA double-strand break processing
DNA synthesis involved in DNA repair
strand displacement
DNA replication
postreplication repair
double-strand break repair
double-strand break repair via nonhomologous end joining
regulation of gene expression by genetic imprinting
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase III promoter
fatty acid biosynthetic process
apoptotic process
cellular response to DNA damage stimulus
DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
chromosome segregation
centrosome cycle
brain development
response to nutrient
intrinsic apoptotic signaling pathway in response to DNA damage
dosage compensation by inactivation of X chromosome
response to ionizing radiation
positive regulation of vascular endothelial growth factor production
positive regulation of gene expression
protein ubiquitination
protein sumoylation
androgen receptor signaling pathway
chromosome breakage
positive regulation of protein ubiquitination
G2 DNA damage checkpoint
response to estradiol
negative regulation of intracellular estrogen receptor signaling pathway
positive regulation of protein import into nucleus, translocation
positive regulation of histone acetylation
negative regulation of histone acetylation
regulation of cell proliferation
regulation of apoptotic process
chordate embryonic development
response to estrogen
regulation of DNA methylation
negative regulation of fatty acid biosynthetic process
positive regulation of DNA repair
positive regulation of angiogenesis
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of centriole replication
positive regulation of histone H3-K4 methylation
negative regulation of histone H3-K4 methylation
negative regulation of histone H3-K9 methylation
positive regulation of histone H3-K9 methylation
protein autoubiquitination
positive regulation of histone H4-K20 methylation
positive regulation of cell cycle arrest
cellular response to tumor necrosis factor
cellular response to indole-3-methanol
protein K6-linked ubiquitination
regulation of signal transduction by p53 class mediator
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of reactive oxygen species metabolic process
positive regulation of histone H3-K9 acetylation
positive regulation of histone H4-K16 acetylation
DNA repair
DNA recombination
lipid metabolic process
fatty acid metabolic process
cell cycle
centrosome duplication
