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BRMS1 Antibody (PA5-78885) in WB
Western blot analysis of BRMS1 in, Lane 1: rat kidney tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: HELA whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour ... View More
产品信息
PA5-78885
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human BRMS1 recombinant protein (Position: D52-D244).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2746001
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: breast cancer metastasis suppressor 1; Breast cancer metastasis-suppressor 1; Breast cancer metastasis-suppressor 1 homolog; BRMS 1
基因别名: AV003220; AW554636; BRMS1
UniProt ID: (Human) Q9HCU9, (Rat) Q5M7T3, (Mouse) Q99N20
Entrez Gene ID: (Human) 25855, (Rat) 293668, (Mouse) 107392
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
apoptotic process
histone deacetylation
negative regulation of NF-kappaB transcription factor activity
negative regulation of transcription, DNA-templated
positive regulation of protein deacetylation
positive regulation of anoikis
regulation of transcription, DNA-templated
cellular process
