Invitrogen

Bcl-X Monoclonal Antibody (2H12)

Bcl-X Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Bcl-X Antibody (MA5-14011) in IHC (P)

Formalin-fixed, paraffin-embedded human Hodgkin’s lymphoma stained with Bcl-X antibody using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of Reedternberg cells.

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产品信息

MA5-14011

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

2-4 µg/mL

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

Assay-dependent

其他PubMed (MISC)

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产品规格

种属反应

Human, Mouse, Pig, Rat

已发表种属

Human, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

2H12

抗原

A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein. This amino acid sequence is shared by human and murine bcl-X protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

AB_10981495

产品详细信息

This antibody reacts with both Bcl-XS (short) and Bcl-XL (long) proteins. The immunogenic sequence (aa 3-14) is conserved in human and mice.

Staining of formalin-fixed paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at room temperature for 20 min. A recommended positive control is Hodgkin's lymphoma.

靶标信息

The protein encoded the BCL-X gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Molecular prognosticators of complex karyotype soft tissue sarcoma outcome: a tissue microarray-based study.

Annals of oncology : official journal of the European Society for Medical Oncology

Lahat G,Tuvin D,Wei C,Wang WL,Pollock RE,Anaya DA,Bekele BN,Corely L,Lazar AJ,Pisters PW,Lev D

MA5-14011 was used in immunohistochemistry - paraffin section to identify prognostic biomarkers for complex karyotype soft tissue sarcoma

Sat May 01 00:00:00 UTC 2010

Bcl-x(L) and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells.

World journal of gastroenterology

Schulze-Bergkamen H,Ehrenberg R,Hickmann L,Vick B,Urbanik T,Schimanski CC,Berger MR,Schad A,Weber A,Heeger S,Galle PR,Moehler M

MA5-14011 was used in western blot to examine the anti-apoptotic role of Bcl-xL and myeloid cell leukaemia-1 in colorectal cancer cells

Sat Jun 28 00:00:00 UTC 2008

Identification of rare Epstein-Barr virus infected memory B cells and plasma cells in non-monomorphic post-transplant lymphoproliferative disorders and the signature of viral signaling.

Haematologica

Shaknovich R,Basso K,Bhagat G,Mansukhani M,Hatzivassiliou G,Murty VV,Buettner M,Niedobitek G,Alobeid B,Cattoretti G

MA5-14011 was used in immunohistochemistry to investigate the role of Epstein-Barr virus infection in the immune response of non-monomorphic post-transplant lymphoproliferative disorders

Sun Oct 01 00:00:00 UTC 2006

Bcl-x(S) induces an NGF-inhibitable cytochrome c release.

Experimental cell research

Lindenboim L,Schlipf S,Kaufmann T,Borner C,Stein R

MA5-14011 was used in immunocytochemistry to elucidate the role of Bcl-x(S) in nerve growth factor-inhibitable apoptosis

Thu Jul 15 00:00:00 UTC 2004

Increased apoptosis in human osteoarthritic cartilage corresponds to reduced cell density and expression of caspase-3.

Arthritis and rheumatism

Sharif M,Whitehouse A,Sharman P,Perry M,Adams M

MA5-14011 was used in immunohistochemistry to investigate cell death in osteoarthritic cartilage and the role of caspase-3 during this process

Sun Feb 01 00:00:00 UTC 2004

Minocycline up-regulates Bcl-2 and protects against cell death in mitochondria.

The Journal of biological chemistry

Wang J,Wei Q,Wang CY,Hill WD,Hess DC,Dong Z

MA5-14011 was used in western blot to study the role of Bcl-2 and mitochrondria in the neuroprotective effects of minocycline

Fri May 07 00:00:00 UTC 2004

Differential expression of bcl-2 family proteins in bladder carcinomas. Relationship with apoptotic rate and survival.

European urology

Korkolopoulou P,Lazaris ACh,Konstantinidou AE,Kavantzas N,Patsouris E,Christodoulou P,Thomas-Tsagli E,Davaris P

MA5-14011 was used in immunohistochemistry to study the role of various bcl-2 family molecules in the regulation of apoptosis and the progression of urothelial cancer.

Fri Mar 01 00:00:00 UTC 2002

生物信息学

蛋白别名: anti-apoptosis regulatory protein; anti-apoptotic Bcl-xL; Apoptosis regulator Bcl-X; B cell lymphoma 2 like; B cell lymphoma like X; B-cell leukemia/lymphoma x; Bcl 2 like 1; bcl x; Bcl xL; BCL XL/S; bcl-2 L1; Bcl-2-like protein 1; bcl-xS; Bcl2-L-1; BCL2-like 1; BclxL; BclXS; DKFZp781P2092; protein phosphatase 1, regulatory subunit 52; RP5-857M17.3

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基因别名: Bcl(X)L; Bcl-X; Bcl-XL; BCL-XL/S; bcl2-L-1; BCL2L; BCL2L1; BCLX; BLC2L; PPP1R52