Invitrogen

Beclin 1 Monoclonal Antibody (2A4)

Beclin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Beclin 1 Antibody (MA5-15825) in IHC (P)

Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and liver cancer tissues (right) using BECN1 monoclonal antibody (Product # MA5-15825) followed with DAB staining.

Beclin 1 Antibody in Flow Cytometry (Flow)

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产品信息

MA5-15825

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

2A4

抗原

Purified recombinant fragment of human BECN1 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11153717

产品详细信息

MA5-15825 targets BECN1 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15825 immunogen is purified recombinant fragment of human BECN1 expressed in E. Coli.

MA5-15825 detects BECN1 which has a predicted molecular weight of approximately 60kDa.

靶标信息

Beclin 1 plays a central role in autophagy. It acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Different PI3K complex forms are believed to play a role in multiple membrane trafficking pathways - PI3KC3-C1 is involved in the initation of autophagosomes and PI3KC3-C2 is involved in the maturation of autophagosomes and endocytosis. Beclin 1 also plays a role in antiviral host defense.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Lipoxin A4 promotes autophagy and inhibits overactivation of macrophage inflammasome activity induced by Pg LPS.

The Journal of international medical research

Zhao J,Geng W,Wan K,Guo K,Xi F,Xu X,Xiong X,Huang X,Liu J,Kuang X

MA5-15825 was used in Western Blot to explore the role of lipoxin A4 on inflammasome and inflammatory activity in macrophages activated by Porphyromonas gingivalis lipopolysaccharide one of the major causative agents of chronic periodontitis.

Mon Feb 01 00:00:00 UTC 2021

Lipoxin A4 promotes autophagy and inhibits overactivation of macrophage inflammasome activity induced by Pg LPS.

The Journal of international medical research

Zhao J,Geng W,Wan K,Guo K,Xi F,Xu X,Xiong X,Huang X,Liu J,Kuang X

Mon Feb 01 00:00:00 UTC 2021

Gambogic acid inhibits proliferation and induces apoptosis of human acute T‑cell leukemia cells by inducing autophagy and downregulating β‑catenin signaling pathway: Mechanisms underlying the effect of Gambogic acid on T‑ALL cells.

Oncology reports

Wang T,Du J,Kong D,Yang G,Zhou Q,You F,Lin Y,Wang Y

MA5-15825 was used in Immunocytochemistry to conclude that GA can inhibit the growth and survival of T‚ALL cells. GA also had antileukemic activity, at least in part, through the downregulation of the Wnt/β-catenin signaling pathway.

Thu Oct 01 00:00:00 UTC 2020

Mechanistic studies of cytotoxic activity of the mesoionic compound MIH 2.4Bl in MCF-7 breast cancer cells.

Oncology letters

de Mascena Costa LA,Debnath D,Harmon AC,de Sousa Araújo S,da Silva Souza HD,de Athayde Filho PF,Wischral A,Adrião Gomes Filho M,Mathis JM

Published figure using Beclin 1 monoclonal antibody (Product # MA5-15825) in Western Blot

Tue Sep 01 00:00:00 UTC 2020

生物信息学

蛋白别名: APG6; ATG6 autophagy related 6 homolog; beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); beclin 1, autophagy related; Beclin-1; Beclin-1-C 35 kDa; Beclin-1-C 37 kDa; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; testis secretory sperm-binding protein Li 215e

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基因别名: ATG6; beclin1; BECN1; GT197; VPS30

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UniProt ID: (Human) Q14457

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Entrez Gene ID: (Human) 8678

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功能

protein binding ubiquitin protein ligase binding phosphatidylinositol 3-kinase binding

参与通路