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Biotin Antibody (53-9895-82) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0036-42) and Anti-Human CD4 Biotin (Product # 13-0048-82) followed by either 0.125 µg of Mouse IgG1 K Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or 0.125 µg of Anti-Biotin Alexa Fluor® 488 (Product # 53-9895-82) (right). Cells in the lymphocyte gate were used for analysis.
产品信息
53-9895-82
产品规格
种属反应
Chemical
已发表种属
Chemical
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
BK-1/39
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2574489
产品详细信息
Description: This BK-1/39 monoclonal antibody reacts with biotin, a B-complex vitamin. As a coenzyme, biotin is involved in the metabolism of fatty acids, amino acids, and gluconeogenesis. In the laboratory, biotin is used to tag molecules of interest for biochemical or cell biological studies. This monoclonal antibody can be used to detect biotinylated proteins and antibodies, particularly if signal amplification is desired.
Applications Reported: This BK-1/39 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This BK-1/39 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained with a biotin-conjugated antibody. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a MW of ~244 Da.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: Vitamin B4
