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Blasticidin S Deaminase Antibody (PA5-143657) in WB
Western blot of Blasticidin S Deaminase in 293 cells mock transfected (lane 1) or transiently transfected with pLenti6/TR lentiviral vector (lanes 2 & 3). Blots were probed with Blasticidin S Deaminase polyclonal antibody (Product # PA5-143657) (lanes 1-3). Molecular weight standards show that bsd is expressed as a 13 kDa band in only the transfected cells.
产品信息
PA5-143657
产品规格
种属反应
Fungi
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase* from Aspergillus Terreus.
偶联物
形式
Liquid
浓度
~0.25 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA, 50% glycerol
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2942885
产品详细信息
The antibody detects a 13 kDa* protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector that expresses the Bsd gene product, but is not detected in mock transfected cells.
靶标信息
Retroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: blasticidin; BSD
