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Blimp-1 Antibody (17-9850-82) in Flow
BALB/c mouse splenocytes were stimulated for 72 hours with LPS (Product # 00-4976-03) and surface stained with with CD45R (B220) Monoclonal Antibody, PE (Product # 12-0452-82). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with 0.5 µg of Rat IgG2a kappa Isotype Control, APC (Product # 17-4321-81) (left) or 0.5 µg of Blimp-1 Monoclonal Antibody, APC (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Pr... View More
产品信息
17-9850-82
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
5E7
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2848296
产品详细信息
Description: This 5E7 monoclonal antibody recognizes mouse B lymphocyte-induced maturation protein-1 (Blimp-1), which is also known as Prdm1. Blimp-1 is a transcriptional repressor with five zinc finger domains. Blimp-1 is expressed by terminally differentiating B and T cells. Blimp-1 is also expressed by some dendritic cells, and some NK cells. This transcriptional repressor is required for B cell differentiation into plasma cells and serves as a critical antagonist of CD4+ follicular helper T cell differentiation. While Blimp-1 is not required for priming and development of T and B cell effector function, it is critical for effector cell terminal differentiation. Blimp-1 promotes effector molecule secretion while suppressing proliferation. Blimp-1 also acts as a transcriptional antagonist of Bcl-6 and inhibits genes that are related to memory cell differentiation.
Applications Reported: This 5E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 5E7 antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols . This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
靶标信息
Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: B lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; BLIMP 1; Blimp-1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; RP1-134E15.1
基因别名: b2b1765Clo; Blimp-1; Blimp1; PRDI-BF1; Prdm1; ZNFPR1A1
UniProt ID: (Mouse) Q60636
Entrez Gene ID: (Mouse) 12142
